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Current Stem Cell Research & Therapy

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ISSN (Print): 1574-888X
ISSN (Online): 2212-3946

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Research Article

Improved Neural Differentiation of Human-induced Pluripotent Stem Cell [hiPSCs] on a Novel Polyurethane-based Scaffold Containing Iron Oxide Nanoparticles [Fe2O3 NPs]

Author(s): Monireh Sadat Hoseinian, Delaram Poormoghadam, Fatemeh Kheirollahzadeh, Arya Mojtahedi, Ali Salimi* and Raheleh Halabian*

Volume 18, Issue 7, 2023

Published on: 26 August, 2022

Page: [993 - 1000] Pages: 8

DOI: 10.2174/1574888X17666220630090418

Price: $65

Abstract

Background: Repair of the nervous system in humans has always been complicated and faced difficulties. Cell transplantation approaches using biocompatible scaffolds might be an attractive therapeutic strategy for neuronal regeneration.

Objective: We designed a cell delivery platform based on polyurethane [PU] and modified it with iron oxide nanoparticles [Fe2O3 NPs] for neural induction of human-induced pluripotent stem cells [hiPSC]. Forskolin, IBMX, and different ratios of FBS were employed to induce neurogenesis of hiPSCs. Neural differentiations were assessed at the level of genes and proteins.

Methods: As was shown by MTT colorimetric assay, the proliferation and viability of SNL 76/7 on PU/ Fe2O3 were superior in comparison with pure PU and Fe2O3. hiPSCs cultured with PU/Fe2O3 exhibited an elevated expression of β3-tubulin, MAP2, NSE, OLIG2, as compared to controls. Furthermore, Acridine Orange staining assured the survival and viability of hiPSCs after 14 days of differentiation.

Results: All in all, our findings pointed out the biocompatibility and positive regulatory effect of PU/Fe2O3 on neural markers.

Conclusion: We believe this scaffold could be a potential candidate for future nerve differentiation applications.

Keywords: Neural differentiation, nanocomposite scaffold, human induced pluripotent stem cell, iron oxide nanoparticles, polyurethanes, Fe2O3.

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