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Current Analytical Chemistry

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ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

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Research Article

Modeling the Influence of Extraction Parameters on the Recovery of Antioxidant Compounds of Microwave Extracted Citrus (Citrus reticulata) Peel by Response Surface Methodology

Author(s): Erryana Martati*, Dwiyani P. Utari and Siti N. Wulan

Volume 18, Issue 8, 2022

Published on: 10 August, 2022

Page: [914 - 925] Pages: 12

DOI: 10.2174/1573411018666220616111017

Price: $65

Abstract

Background: Microwave-assisted extraction (MAE) is a green extraction technology that saves both in energy and time and that bioactive compounds can be extracted without loss as much as in traditional extraction methods.

Objectives: This study aims to optimize the MAE process for maximizing the recovery of antioxidant compounds of flavonoids and the activity of radical scavenging of DPPH (2,2-diphenyl-1-picrylhydrazyl) from citrus peels (CP).

Methods: The modeling used Box-Behnken Design (BBD) experimental design for optimizing three extraction parameters of time (5, 15, and 25 min), ethanol concentration (50%, 70%, and 90%), and extraction temperature (55, 65, and 75°C). UHPLC-MS analysis was performed to identify the qualitative flavonoid content in the extract.

Results: Our results showed that the optimum MAE conditions that maximize the total flavonoid content and antioxidant activity of the CP extract were extraction time of 16.87 min, 50% ethanol, and temperature at 75°C. The obtained CP extract had a TFC of 14.10 mg QE/g and antioxidant activity of 51.52%, which was in line with the predicted values. Flavonoids of neohesperidin, naringin, hesperidin, narirutin, and nobiletin were identified in the extract of CP.

Conclusion: The ethanolic extract of CP was confirmed to have high flavonoids content with potent antioxidant activity.

Keywords: Citrus peel, microwave-assisted extraction, flavonoid, antioxidant, response surface method, respiratory syncytial virus.

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