CRISPR/Cas9 定向碱基编辑的最新进展

Title:Recent Advances in CRISPR/Cas9 Directed Base Editing

Volume: 21 Issue: 4

关键词: 基于 CRISPR 的技术、基因组、靶向碱基编辑、人类遗传疾病、点突变、碱基编辑工具。

摘要: Recently, CRISPR-based techniques have significantly improved our ability to make desired changes and regulations in various genomes. Among them, targeted base editing is one of the most powerful techniques in making precise genomic editing. Base editing enabled the irreversible conversion of a specific single DNA base, from C to T or and from A to G, in desired genomic loci. This technique has important implications in the study of human genetic diseases, considering that many of them resulted from point mutations. More importantly, the high efficiency of these editing tools also provided great promise in clinical applications. In this review, we discuss the recent progress and challenges of base editing tools.

Cite this article as:

Recent Advances in CRISPR/Cas9 Directed Base Editing, Current Gene Therapy 2021; 21 (4) . https://dx.doi.org/10.2174/1566523221666210322090638