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Combinatorial Chemistry & High Throughput Screening

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ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

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Research Article

Compositional Analysis and Antioxidant Activities of Thymus pubescens Essential Oil from Iran

Author(s): Bahman Nickavar* and Elaheh Malekitabar

Volume 25, Issue 2, 2022

Published on: 29 December, 2020

Page: [252 - 258] Pages: 7

DOI: 10.2174/1386207323666201230091042

Price: $65

Abstract

Background: The genus Thymus is one of the well-known members of the Lamiaceae, comprising about 215 species. It is a polymorphic genus in this family. Therefore, possible differences in chemical composition among polymorphs may result in differences in their biological activities.

Objective: This study was designed to specify chemical composition in order to assess antioxidant activities and to characterize active constituents of essential oil of Thymus pubescens.

Methods: Essential oil was collected by hydrodistillation and was analyzed using GC-MS and GCFID. Antioxidant activities were evaluated by ABTS radical cation scavenging and β-carotene bleaching inhibition methods. Detection and identification of antioxidant components were performed by TLC-bioautography assay using ABTS•+ reagent.

Results: GC analyses indicated the presence of 39 components, accounting for 96.64% of total constituents, with thymol (38.67%), γ-terpinene (7.46%), and p-cymene (5.54%) as the main components. The IC50 values for antioxidant activities were equal to 1.15 (1.05 – 1.25) μg/mL and 0.45 (0.33 – 0.62) μg/mL for ABTS•+ scavenging and β-carotene bleaching inhibition tests, respectively. TLC-bioautography of oil resulted in the identification of thymol as the major antioxidant compound.

Conclusion: Essential oil exhibited very potent and significant antioxidant activities correlated well with increasing dose in vitro.

Keywords: Antioxidant activity, bioautography, chemical composition, essential oil, Lamiaceae, Thymus pubescens.

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[1]
Stahl-Biskup, E.; Sáez, F. Thyme, 1st ed; CRC Press: London, 2002.
[http://dx.doi.org/10.4324/9780203216859]
[2]
Mozaffarian, V. A Dictionary of Iranian Plants Names, 1st ed; Farhang Moaser Publishers: Tehran, 1998.
[3]
Nazemiyeh, H.; Lotfipoor, F.; Delazar, A.; Razavi, S.M.; Asnaashari, S.; Kasebi, N.; Talebpour, A.H.; Nahar, L.; Sarker, S.D. Chemical composition, and antibacterial and free radical scavenging activities of the essential oils of a citronellol producing new chemotype of Thymus pubescens Boiss. & Kotschy ex Celak. Rec. Nat. Prod., 2011, 5(3), 184-192.
[4]
Abousaber, M.; Hadjakhoondi, A.; Shafiee, A. Composition of the essential oil of Thymus pubescens Boiss. et Kotschy ex Celak and Thymus fedtschenkoi Ronniger from Iran. J. Essent. Oil Res., 2002, 14(3), 154-155.
[http://dx.doi.org/10.1080/10412905.2002.9699808]
[5]
Sefidkon, F.; Askari, F.; Ghorbanli, M. Essential oil composition of Thymus pubescens Boiss. et Kotschy ex Celak from Iran. J. Essent. Oil Res., 2002, 14(2), 116-117.
[http://dx.doi.org/10.1080/10412905.2002.9699789]
[6]
Mahmoudi, M.; Morteza-Semnani, K.; Mojra, E. Anti-inflammatory and antinociceptive activity of Thymus pubescens extract. Fitoterapia, 2008, 79(5), 361-365.
[http://dx.doi.org/10.1016/j.fitote.2008.04.004] [PMID: 18504076]
[7]
Mohseni, S.; Rad, A.S. Determination of compositions of Thymus pubescens; The comparison of different dolvents towards extraction. Iran. J. Sci. Technol. Trans. A Sci., 2018, 42(4), 1923-1928.
[http://dx.doi.org/10.1007/s40995-017-0159-3]
[8]
Morteza-Semnani, K.; Rostami, B.; Akbarzadeh, M. Essential oil composition of Thymus kotschyanus and Thymus pubescens from Iran. J. Essent. Oil Res., 2006, 18(3), 272-274.
[http://dx.doi.org/10.1080/10412905.2006.9699085]
[9]
Hashemi-Moghaddam, H.; Mohammadhosseini, M.; Basiri, M. Optimization of microwave assisted hydrodistillationon chemical compositions of the essential oils from the aerial parts of Thymus pubescens and comparison with conventional hydrodistllation. J. Essent. Oil-Bear. Plants, 2015, 18(4), 884-893.
[10]
Baser, K.H.C.; Özek, T.; Kürkcüoglu, M.; Yildiz, B. Composition of the essential oils of Thymus leucostomus Hausskn. et Velen var. gypsaceus Jalas and Thymus pubescens Boiss. et Kotschy ex Celak var. cratericola Jalas. J. Essent. Oil Res., 1999, 11(6), 776-778.
[http://dx.doi.org/10.1080/10412905.1999.9712018]
[11]
Zehra Küçükbay, F.; Kuyumcu, E.; Çelen, S.; Azaz, A.D.; Arabaci, T. Chemical composition of the essential oils of three Thymus Taxa from Turkey with antimicrobial and antioxidant activities. Rec. Nat. Prod., 2014, 8(2), 110-120.
[12]
Mahboubi, M.; Heidarytabar, R.; Mahdizadeh, E.; Hosseini, H. Antimicrobial activity and chemical composition of Thymus species and Zataria multiflora essential oils. Agric. Nat. Resour. (Bangk.), 2017, 51(5), 395-401.
[http://dx.doi.org/10.1016/j.anres.2018.02.001]
[13]
Rasooli, I.; Mirmostafa, S.A. Antibacterial properties of Thymus pubescens and Thymus serpyllum essential oils. Fitoterapia, 2002, 73(3), 244-250.
[http://dx.doi.org/10.1016/S0367-326X(02)00064-3] [PMID: 12048019]
[14]
Salehi, B.; Abu-Darwish, M.S.; Tarawneh, A.H.; Cabral, C.; Gadetskaya, A.V.; Salgueiro, L.; Hosseinabadi, T.; Rajabi, S.; Chanda, W.; Sharifi-Rad, M.; Mulaudzi, R.B.; Ayatollahi, S.A.; Kobarfard, F.; Arserim-Uçar, D.K.; Sharifi-Rad, J.; Ata, A.; Baghalpour, N.; Contreras, M.M. Thymus spp. plants - Food applications and phytopharmacy properties. Trends Food Sci. Technol., 2019, 85, 287-306.
[http://dx.doi.org/10.1016/j.tifs.2019.01.020]
[15]
Trindade, H.; Pedro, L.G.; Figueiredo, A.C.; Barroso, J.G. Chemotypes and terpene synthase genes in Thymus genus: State of the art. Ind. Crops Prod., 2018, 124, 530-547.
[http://dx.doi.org/10.1016/j.indcrop.2018.08.021]
[16]
Jugreet, B.S.; Suroowan, S.; Rengasamy, R.R.K.; Mahomoodally, M.F. Chemistry, bioactivities, mode of action and industrial applications of essential oils. Trends Food Sci. Technol., 2020, 101, 89-105.
[http://dx.doi.org/10.1016/j.tifs.2020.04.025]
[17]
Spisni, E.; Petrocelli, G.; Imbesi, V.; Spigarelli, R.; Azzinnari, D.; Donati Sarti, M.; Campieri, M.; Valerii, M.C. Antioxidant, anti-inflammatory, and microbial-modulating activities of essential oils: Implications in colonic pathophysiology. Int. J. Mol. Sci., 2020, 21(11), 1-27.
[http://dx.doi.org/10.3390/ijms21114152] [PMID: 32532055]
[18]
Li, X.; He, T.; Wang, X.; Shen, M.; Yan, X.; Fan, S.; Wang, L.; Wang, X.; Xu, X.; Sui, H.; She, G. Traditional uses, chemical constituents and biological activities of plants from the genus Thymus. Chem. Biodivers., 2019, 16(9), e1900254.
[http://dx.doi.org/10.1002/cbdv.201900254] [PMID: 31381251]
[19]
Nickavar, B.; Adeli, A.; Nickavar, A. TLC-bioautography and GC-MS analyses for detection and identification of antioxidant constituents of Trachyspermum copticum essential oil. Iran. J. Pharm. Res., 2014, 13(1), 127-133.
[PMID: 24734063]
[20]
Nickavar, B.; Adeli, A.; Nickavar, A. Analyses of the essential oil from Bunium persicum fruit and its antioxidant constituents. J. Oleo Sci., 2014, 63(7), 741-746.
[http://dx.doi.org/10.5650/jos.ess13168] [PMID: 24919477]
[21]
Danciu, V.; Hosu, A.; Cimpoiu, C. Comparative evaluation of antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) methods. J. Planar Chromatogr. Mod. TLC, 2016, 29(4), 306-309.
[http://dx.doi.org/10.1556/1006.2016.29.4.9]
[22]
Ćavar Zeljković, S.; Maksimović, M. Chemical composition and bioactivity of essential oil from Thymus species in Balkan Peninsula. Phytochem. Rev., 2015, 14(3), 335-352.
[http://dx.doi.org/10.1007/s11101-014-9378-9]
[23]
Bradesi, P.; Tomi, F.; Casanova, J.; Costa, J.; Bemardini, A.F. Chemical composition of myrtle leaf essential oil from Corsica (France). J. Essent. Oil Res., 1997, 9(3), 283-288.
[http://dx.doi.org/10.1080/10412905.1997.10554245]
[24]
Figueiredo, A.C.; Barroso, J.G.; Pedro, L.G.; Scheffer, J.J.C. Factors affecting secondary metabolite production in plants: Volatile components and essential oils. Flavour Fragrance J., 2008, 23(4), 213-226.
[http://dx.doi.org/10.1002/ffj.1875]
[25]
Tohidi, B.; Rahimmalek, M.; Trindade, H. Review on essential oil, extracts composition, molecular and phytochemical properties of Thymus species in Iran. Ind. Crops Prod., 2019, 134, 89-99.
[http://dx.doi.org/10.1016/j.indcrop.2019.02.038]
[26]
Aktumsek, A.; Zengin, G.; Guler, G.O.; Cakmak, Y.S.; Duran, A. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food Chem. Toxicol., 2013, 55, 290-296.
[http://dx.doi.org/10.1016/j.fct.2013.01.018] [PMID: 23357566]
[27]
Öztürk, M. Anticholinesterase and antioxidant activities of Savoury (Satureja thymbra L.) with identified major terpenes of the essential oil. Food Chem., 2012, 134(1), 48-54.
[http://dx.doi.org/10.1016/j.foodchem.2012.02.054] [PMID: 23265454]
[28]
Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 1999, 26(9-10), 1231-1237.
[http://dx.doi.org/10.1016/S0891-5849(98)00315-3] [PMID: 10381194]
[29]
Marco, G.J. A rapid method for evaluation of antioxidants. J. Am. Oil Chem. Soc., 1968, 45(9), 594-598.
[http://dx.doi.org/10.1007/BF02668958]
[30]
Nickavar, B.; Esbati, N. Evaluation of the antioxidant capacity and phenolic content of three Thymus species. J. Acupunct. Meridian Stud., 2012, 5(3), 119-125.
[http://dx.doi.org/10.1016/j.jams.2012.03.003] [PMID: 22682273]

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