CESI-MS对抗体配体缀合物的多水平表征

Title:Multilevel Characterization of Antibody-Ligand Conjugates by CESI-MS

Volume: 20 Issue: 10

关键词: 抗体药物缀合物，蛋白质小分子缀合物，CE-MS，化学计量学，表征，未修饰的多肽。

摘要:

Aim: To demonstrate the capabilities of our new capillary electrophoresis – mass spectrometry method, which facilitates highly accurate relative quantitation of modification site occupancy of antibody-ligand (e.g., antibody-drug) conjugates.

Background: Antibody-drug conjugates play important roles in medical discovery for imaging and therapeutic intervention. The localization and stoichiometry of the conjugation can affect the orientation, selectivity, specificity, and strength of molecular interactions, influencing biochemical function.

Objective: To demonstrate the option to analyze the localization and stoichiometry of antibody-ligand conjugates by using essentially the same method at all levels including ligand infusion, peptide mapping, as well as reduced and intact protein analysis.

Materials and Methods: Capillary electrophoresis coupled with electrospray ionization mass spectrometry was used to analyze the antibody-ligand conjugates.

Results: We identified three prevalent ligand conjugation sites with estimated stoichiometries of 73, 14, and 6% and an average ligand-antibody ratio of 1.37, illustrating the capabilities of CE-ESI-MS for rapid and efficient characterization of antibody-drug conjugates.

Conclusion: The developed multilevel analytical method offers a comprehensive way to determine the localization and stoichiometry of antibody-drug conjugates for molecular medicinal applications. In addition, a significant advantage of the reported approach is the small, hydrophilic, unmodified peptides well separated from the neutrals, which is not common with other liquid phase separation methods such as LC.

Cite this article as:

Multilevel Characterization of Antibody-Ligand Conjugates by CESI-MS, Current Molecular Medicine 2020; 20 (10) . https://dx.doi.org/10.2174/1566524020666200415095830