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Research Article

小鼠胚胎植入不需要子宫Dnmt3a

卷 20, 期 8, 2020

页: [633 - 642] 页: 10

弟呕挨: 10.2174/1566524020666200225115526

价格: $65

摘要

背景：近年来的研究表明，子宫内膜DNA甲基化对妊娠早期胚胎着床至关重要。Dnmt3a是DNA甲基化的关键酶之一，该阶段在子宫内膜中有规律表达。 目的和方法：在本研究中，我们使用孕酮受体cre (Pgrcre)对子宫Dnmt3a进行条件消融，以确定Dnmt3a在女性生殖中的生理作用。 结果：我们发现，在妊娠早期，在Dnmt3aloxP/loxP Pgrcre/+ (cKO)中的卵巢功能没有明显改变，胚胎着床位点的数量也没有明显变化。Western blotting和免疫组化结果显示雌激素受体α(ERα)和黏液素1 (Muc1)的表达和位置均无差异。虽然蜕膜标记物基质金属蛋白酶-2 (Mmp2)、基质金属蛋白酶-9(Mmp9)和骨形态发生蛋白-2 (Bmp2)在Dnmt3a cKO雌性小鼠中的表达略有降低，但蜕膜过程中小鼠子宫的大体形态未受显著影响。人工诱导脱膜模型时，Dnmt3a cKO中目测形态及子宫重量也无明显差异。最后，一项持续的繁殖研究表明，Dnmt3a cKO雌性小鼠的生育能力没有明显改变。 结论：

关键词: Dnmt3a，子宫内膜容受性，脱膜，条件性敲除，胚胎着床，子宫。

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