Login Register Cart 0
Generic placeholder image

Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Mini-Review Article

A Comprehensive Guide to the Commercial Baculovirus Expression Vector Systems for Recombinant Protein Production

Author(s): Vibhor Mishra*

Volume 27, Issue 6, 2020

Page: [529 - 537] Pages: 9

DOI: 10.2174/0929866526666191112152646

Price: $65

Abstract

The Baculovirus Expression Vector System (BEVS) is a workhorse for recombinant protein expression for over thirty-five years. Ever since it was first used to overexpress the human IFN-β protein, the system has been engineered and modified several times for quick and easy expression and scale-up of the recombinant proteins. Multiple gene assemblies performed on the baculovirus genome using synthetic biology methods lead to optimized overexpression of the multiprotein complexes. Nowadays, several commercially available BEVS platforms offer a variety of customizable features, and often it is confusing which one to choose for a novice user. This short review is intended to be a one-stop guide to the commercially available baculovirus technology for heterologous protein expression in the insect cells, which users can refer to choose from popular and desirable BEVS products or services.

Keywords: Baculovirus, insect cell, BEVS, protein expression, MultiBac, AcMNPV.

« Previous Next »
Graphical Abstract

[1]
Irons, S.L.; Chambers, A.C.; Lissina, O.; King, L.A.; Possee, R.D. Protein production using the baculovirus expression system., Curr. Protoc. Protein Sci., 2018, 91, 5.5.1-5.5.22.
[http://dx.doi.org/10.1002/cpps.45]
[2]
Kost, T.A.; Condreay, J.P.; Jarvis, D.L. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat. Biotechnol., 2005, 23(5), 567-575.
[http://dx.doi.org/10.1038/nbt1095] [PMID: 15877075]
[3]
van Oers, M.M.; Pijlman, G.P.; Vlak, J.M. Thirty years of baculovirus-insect cell protein expression: from dark horse to mainstream technology. J. Gen. Virol., 2015, 96(Pt 1), 6-23.
[http://dx.doi.org/10.1099/vir.0.067108-0] [PMID: 25246703]
[4]
Jarvis, D.L. Baculovirus-insect cell expression systems. Methods Enzymol., 2009, 463, 191-222.
[http://dx.doi.org/10.1016/S0076-6879(09)63014-7] [PMID: 19892174]
[5]
Jarvis, D.L. Recombinant protein expression in baculovirus-infected insect cells. Methods Enzymol., 2014, 536, 149-163.
[http://dx.doi.org/10.1016/B978-0-12-420070-8.00013-1] [PMID: 24423274]
[6]
Berger, I.; Mayr, L.M. Protein production for structural biology: new solutions to new challenges. Curr. Opin. Struct. Biol., 2013, 23(3), 317-318.
[http://dx.doi.org/10.1016/j.sbi.2013.05.004] [PMID: 23735677]
[7]
Barford, D.; Takagi, Y.; Schultz, P.; Berger, I. Baculovirus expression: tackling the complexity challenge. Curr. Opin. Struct. Biol., 2013, 23(3), 357-364.
[http://dx.doi.org/10.1016/j.sbi.2013.03.009] [PMID: 23628287]
[8]
Smith, G.E.; Summers, M.D.; Fraser, M.J. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell. Biol., 1983, 3(12), 2156-2165.
[http://dx.doi.org/10.1128/MCB.3.12.2156] [PMID: 6318086]
[9]
Berger, I.; Poterszman, A. Baculovirus expression: old dog, new tricks. Bioengineered, 2015, 6(6), 316-322.
[http://dx.doi.org/10.1080/21655979.2015.1104433] [PMID: 26488462]
[10]
Kelly, B.J.; King, L.A.; Possee, R.D. Introduction to baculovirus molecular biology. Methods Mol. Biol., 2016, 1350, 25-50.
[http://dx.doi.org/10.1007/978-1-4939-3043-2_2] [PMID: 26820852]
[11]
Kost, T.A.; Kemp, C.W. Fundamentals of baculovirus expression and applications. Adv. Exp. Med. Biol., 2016, 896, 187-197.
[http://dx.doi.org/10.1007/978-3-319-27216-0_12] [PMID: 27165326]
[12]
Bieniossek, C.; Imasaki, T.; Takagi, Y.; Berger, I. MultiBac: expanding the research toolbox for multiprotein complexes. Trends Biochem. Sci., 2012, 37(2), 49-57.
[http://dx.doi.org/10.1016/j.tibs.2011.10.005] [PMID: 22154230]
[13]
Gupta, K.; Tölzer, C.; Sari-Ak, D.; Fitzgerald, D.J.; Schaffitzel, C.; Berger, I. MultiBac: Baculovirus-mediated multigene DNA cargo delivery in insect and mammalian cells. Viruses, 2019, 11(3)E198
[http://dx.doi.org/10.3390/v11030198] [PMID: 30813511]
[14]
Pelosse, M.; Crocker, H.; Gorda, B.; Lemaire, P.; Rauch, J.; Berger, I. MultiBac: from protein complex structures to synthetic viral nanosystems. BMC Biol., 2017, 15(1), 99.
[http://dx.doi.org/10.1186/s12915-017-0447-6] [PMID: 29084535]
[15]
Sari, D.; Gupta, K.; Thimiri Govinda Raj, D.B.; Aubert, A.; Drncová, P.; Garzoni, F.; Fitzgerald, D.; Berger, I. The MultiBac baculovirus/insect cell expression vector system for producing complex protein biologics. Adv. Exp. Med. Biol., 2016, 896, 199-215.
[http://dx.doi.org/10.1007/978-3-319-27216-0_13] [PMID: 27165327]
[16]
Perrakis, A.; Musacchio, A.; Cusack, S.; Petosa, C. Investigating a macromolecular complex: the toolkit of methods. J. Struct. Biol., 2011, 175(2), 106-112.
[http://dx.doi.org/10.1016/j.jsb.2011.05.014] [PMID: 21620973]
[17]
Almo, S.C.; Garforth, S.J.; Hillerich, B.S.; Love, J.D.; Seidel, R.D.; Burley, S.K. Protein production from the structural genomics perspective: achievements and future needs. Curr. Opin. Struct. Biol., 2013, 23(3), 335-344.
[http://dx.doi.org/10.1016/j.sbi.2013.02.014] [PMID: 23642905]
[18]
Abdulrahman, W.; Uhring, M.; Kolb-Cheynel, I.; Garnier, J.M.; Moras, D.; Rochel, N.; Busso, D.; Poterszman, A. A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies. Anal. Biochem., 2009, 385(2), 383-385.
[http://dx.doi.org/10.1016/j.ab.2008.10.044] [PMID: 19061853]
[19]
Hu, N.J.; Rada, H.; Rahman, N.; Nettleship, J.E.; Bird, L.; Iwata, S.; Drew, D.; Cameron, A.D.; Owens, R.J. GFP-based expression screening of membrane proteins in insect cells using the baculovirus system. Methods Mol. Biol., 2015, 1261, 197-209.
[http://dx.doi.org/10.1007/978-1-4939-2230-7_11] [PMID: 25502201]
[20]
Osz-Papai, J.; Radu, L.; Abdulrahman, W.; Kolb-Cheynel, I.; Troffer-Charlier, N.; Birck, C.; Poterszman, A. Insect cells-baculovirus system for the production of difficult to express proteins. Methods Mol. Biol., 2015, 1258, 181-205.
[http://dx.doi.org/10.1007/978-1-4939-2205-5_10] [PMID: 25447865]
[21]
Unger, T.; Peleg, Y. Recombinant protein expression in the baculovirus-infected insect cell system. Methods Mol. Biol., 2012, 800, 187-199.
[http://dx.doi.org/10.1007/978-1-61779-349-3_13] [PMID: 21964790]
[22]
Ng, G.Y.; George, S.R.; Zastawny, R.L.; Caron, M.; Bouvier, M.; Dennis, M.; O’Dowd, B.F. Human serotonin1B receptor expression in Sf9 cells: phosphorylation, palmitoylation, and adenylyl cyclase inhibition. Biochemistry, 1993, 32(43), 11727-11733.
[http://dx.doi.org/10.1021/bi00094a032] [PMID: 8218242]
[23]
Zhao, Y.; Sane, D.C. Expression of a recombinant baculovirus for vitronectin in insect cells: purification, characterization of post-translational modifications and functional studies of the recombinant protein. Arch. Biochem. Biophys., 1993, 304(2), 434-442.
[http://dx.doi.org/10.1006/abbi.1993.1372] [PMID: 7688496]
[24]
Linder, M.E.; Middleton, P.; Hepler, J.R.; Taussig, R.; Gilman, A.G.; Mumby, S.M. Lipid modifications of G proteins: alpha subunits are palmitoylated. Proc. Natl. Acad. Sci. USA, 1993, 90(8), 3675-3679.
[http://dx.doi.org/10.1073/pnas.90.8.3675] [PMID: 8475115]
[25]
Altmann, F.; Staudacher, E.; Wilson, I.B.; März, L. Insect cells as hosts for the expression of recombinant glycoproteins. Glycoconj. J., 1999, 16(2), 109-123.
[http://dx.doi.org/10.1023/A:1026488408951] [PMID: 10612411]
[26]
Kanai, Y.; Athmaram, T.N.; Stewart, M.; Roy, P. Multiple large foreign protein expression by a single recombinant baculovirus: a system for production of multivalent vaccines. Protein Expr. Purif., 2013, 91(1), 77-84.
[http://dx.doi.org/10.1016/j.pep.2013.07.005] [PMID: 23872366]
[27]
Mahajan, P.; Strain-Damerell, C.; Gileadi, O.; Burgess-Brown, N.A. Medium-throughput production of recombinant human proteins: protein production in insect cells. Methods Mol. Biol., 2014, 1091, 95-121.
[http://dx.doi.org/10.1007/978-1-62703-691-7_6] [PMID: 24203326]
[28]
Possee, R.D.; Hitchman, R.B.; Richards, K.S.; Mann, S.G.; Siaterli, E.; Nixon, C.P.; Irving, H.; Assenberg, R.; Alderton, D.; Owens, R.J.; King, L.A. Generation of baculovirus vectors for the high-throughput production of proteins in insect cells. Biotechnol. Bioeng., 2008, 101(6), 1115-1122.
[http://dx.doi.org/10.1002/bit.22002] [PMID: 18781697]
[29]
Yang, J.P. Small-scale production of recombinant proteins using the baculovirus expression vector system. Methods Mol. Biol., 2016, 1350, 225-239.
[http://dx.doi.org/10.1007/978-1-4939-3043-2_10] [PMID: 26820860]
[30]
Kaba, S.A.; Salcedo, A.M.; Wafula, P.O.; Vlak, J.M.; van Oers, M.M. Development of a chitinase and v-cathepsin negative bacmid for improved integrity of secreted recombinant proteins. J. Virol. Methods, 2004, 122(1), 113-118.
[http://dx.doi.org/10.1016/j.jviromet.2004.07.006] [PMID: 15488628]
[31]
Kitts, P.A.; Ayres, M.D.; Possee, R.D. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res., 1990, 18(19), 5667-5672.
[http://dx.doi.org/10.1093/nar/18.19.5667] [PMID: 2216760]
[32]
Blevins, T.; Podicheti, R.; Mishra, V.; Marasco, M.; Wang, J.; Rusch, D.; Tang, H.; Pikaard, C.S. Identification of Pol IV and RDR2-dependent precursors of 24 nt siRNAs guiding de novo DNA methylation in Arabidopsis. eLife, 2015, 4e09591
[http://dx.doi.org/10.7554/eLife.09591] [PMID: 26430765]
[33]
Singh, J.; Mishra, V.; Wang, F.; Huang, H.Y.; Pikaard, C.S. Reaction mechanisms of Pol IV, RDR2, and DCL3 Drive RNA channeling in the siRNA-directed DNA methylation pathway. Mol. Cell, 2019, 75(3), 576-589.e5.
[34]
Abdulrahman, W.; Radu, L.; Garzoni, F.; Kolesnikova, O.; Gupta, K.; Osz-Papai, J.; Berger, I.; Poterszman, A. The production of multiprotein complexes in insect cells using the baculovirus expression system. Methods Mol. Biol., 2015, 1261, 91-114.
[http://dx.doi.org/10.1007/978-1-4939-2230-7_5] [PMID: 25502195]
[35]
Lyons, J.A.; Shahsavar, A.; Paulsen, P.A.; Pedersen, B.P.; Nissen, P. Expression strategies for structural studies of eukaryotic membrane proteins. Curr. Opin. Struct. Biol., 2016, 38, 137-144.
[http://dx.doi.org/10.1016/j.sbi.2016.06.011] [PMID: 27362979]
[36]
Bieniossek, C.; Papai, G.; Schaffitzel, C.; Garzoni, F.; Chaillet, M.; Scheer, E.; Papadopoulos, P.; Tora, L.; Schultz, P.; Berger, I. The architecture of human general transcription factor TFIID core complex. Nature, 2013, 493(7434), 699-702.
[http://dx.doi.org/10.1038/nature11791] [PMID: 23292512]
[37]
Trowitzsch, S.; Viola, C.; Scheer, E.; Conic, S.; Chavant, V.; Fournier, M.; Papai, G.; Ebong, I.O.; Schaffitzel, C.; Zou, J.; Haffke, M.; Rappsilber, J.; Robinson, C.V.; Schultz, P.; Tora, L.; Berger, I. Cytoplasmic TAF2-TAF8-TAF10 complex provides evidence for nuclear holo-TFIID assembly from preformed submodules. Nat. Commun., 2015, 6, 6011.
[http://dx.doi.org/10.1038/ncomms7011] [PMID: 25586196]
[38]
Abdulrahman, W.; Iltis, I.; Radu, L.; Braun, C.; Maglott-Roth, A.; Giraudon, C.; Egly, J.M.; Poterszman, A. ARCH domain of XPD, an anchoring platform for CAK that conditions TFIIH DNA repair and transcription activities. Proc. Natl. Acad. Sci. USA, 2013, 110(8), E633-E642.
[http://dx.doi.org/10.1073/pnas.1213981110] [PMID: 23382212]
[39]
Toste Rêgo, A.; da Fonseca, P.C.A. Characterization of fully recombinant human 20S and 20S-PA200 proteasome complexes. Mol. Cell, 2019, 76(1), 138-140.
[40]
Schöppe, J.; Ehrenmann, J.; Klenk, C.; Rucktooa, P.; Schütz, M.; Doré, A.S.; Plückthun, A. Crystal structures of the human neurokinin 1 receptor in complex with clinically used antagonists. Nat. Commun., 2019, 10(1), 17.
[http://dx.doi.org/10.1038/s41467-018-07939-8] [PMID: 30604743]
[41]
Imasaki, T.; Calero, G.; Cai, G.; Tsai, K.L.; Yamada, K.; Cardelli, F.; Erdjument-Bromage, H.; Tempst, P.; Berger, I.; Kornberg, G.L.; Asturias, F.J.; Kornberg, R.D.; Takagi, Y. Architecture of the Mediator head module. Nature, 2011, 475(7355), 240-243.
[http://dx.doi.org/10.1038/nature10162] [PMID: 21725323]
[42]
Saarenpää, T.; Jaakola, V.P.; Goldman, A. Baculovirus-mediated expression of GPCRs in insect cells. Methods Enzymol., 2015, 556, 185-218.
[http://dx.doi.org/10.1016/bs.mie.2014.12.033] [PMID: 25857783]
[43]
Stolt-Bergner, P.; Benda, C.; Bergbrede, T.; Besir, H.; Celie, P.H.N.; Chang, C.; Drechsel, D.; Fischer, A.; Geerlof, A.; Giabbai, B.; van den Heuvel, J.; Huber, G.; Knecht, W.; Lehner, A.; Lemaitre, R.; Nordén, K.; Pardee, G.; Racke, I.; Remans, K.; Sander, A.; Scholz, J.; Stadnik, M.; Storici, P.; Weinbruch, D.; Zaror, I.; Lua, L.H.L.; Suppmann, S. Baculovirus-driven protein expression in insect cells: A benchmarking study. J. Struct. Biol., 2018, 203(2), 71-80.
[http://dx.doi.org/10.1016/j.jsb.2018.03.004] [PMID: 29545204]
[44]
Koehler, C.; Sauter, P.F.; Wawryszyn, M.; Girona, G.E.; Gupta, K.; Landry, J.J.; Fritz, M.H.; Radic, K.; Hoffmann, J.E.; Chen, Z.A.; Zou, J.; Tan, P.S.; Galik, B.; Junttila, S.; Stolt-Bergner, P.; Pruneri, G.; Gyenesei, A.; Schultz, C.; Biskup, M.B.; Besir, H.; Benes, V.; Rappsilber, J.; Jechlinger, M.; Korbel, J.O.; Berger, I.; Braese, S.; Lemke, E.A. Genetic code expansion for multiprotein complex engineering. Nat. Methods, 2016, 13(12), 997-1000.
[http://dx.doi.org/10.1038/nmeth.4032] [PMID: 27749839]
[45]
Trowitzsch, S.; Palmberger, D.; Fitzgerald, D.; Takagi, Y.; Berger, I. MultiBac complexomics. Expert Rev. Proteomics, 2012, 9(4), 363-373.
[http://dx.doi.org/10.1586/epr.12.32] [PMID: 22967074]
[46]
Fitzgerald, D.J.; Berger, P.; Schaffitzel, C.; Yamada, K.; Richmond, T.J.; Berger, I. Protein complex expression by using multigene baculoviral vectors. Nat. Methods, 2006, 3(12), 1021-1032.
[http://dx.doi.org/10.1038/nmeth983] [PMID: 17117155]
[47]
Berger, I.; Fitzgerald, D.J.; Richmond, T.J. Baculovirus expression system for heterologous multiprotein complexes. Nat. Biotechnol., 2004, 22(12), 1583-1587.
[http://dx.doi.org/10.1038/nbt1036] [PMID: 15568020]
[48]
Vaughn, J.L.; Goodwin, R.H.; Tompkins, G.J.; McCawley, P. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro, 1977, 13(4), 213- 217.
[http://dx.doi.org/10.1007/BF02615077 ] [PMID: 68913]
[49]
Wickham, T.J.; Davis, T.; Granados, R.R.; Shuler, M.L.; Wood, H.A. Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system. Biotechnol. Prog., 1992, 8(5), 391-396.
[http://dx.doi.org/10.1021/bp00017a003] [PMID: 1369220]

Rights & Permissions Print Cite
Article Metrics
50
5
© 2024 Bentham Science Publishers | Privacy Policy