Abstract
Abiotic stresses have contributed to the generation of reactive oxygen species called as free radicals which are highly toxic to the organism. Free radicals may be evaluated either explicitly or inadvertently after the production of oxidative byproducts of nucleic acids, proteins or lipids, a method also known as fingerprinting. Though the approaches for analyzing such reactive intermediates have been thoroughly studied; we concentrated primarily on recent implementations of these techniques to quantify free radicals and different candidate biomarkers of oxidative stress such as nitrotyrosine, isoprostane, etc. Further, the various biochemical approaches along with the conventional methods are also discussed for the evaluation of antioxidant activity of natural products.
Keywords: Antioxidants, Biochemical approaches, Biomarkers, Oxidative stress.
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