摘要
背景:基于自发拉曼散射效应的光谱成像可以提供与分子振动频带相关的独特指纹信息。拉曼光谱成像(RSI)具有化学特异性高、无创检测能力、对水的敏感性低、无需特殊样品预处理等优点,已成为生物医学和药物化学领域的一种有价值的工具。 方法:实现RSI有三种方法,即点扫描、线扫描和宽视场RSI。点扫描可以实现目标样品的二维和三维成像。高光谱分辨率、全光谱范围和共焦特性使这一技术非常有吸引力。然而,基于点扫描的RSI是一个耗时的过程,可能需要几个小时来绘制一个小区域。线扫描RSI是点扫描方法的扩展,成像速度提高300-600倍。在宽场RSI中,激光直接照亮整个感兴趣的区域,然后收集所有的图像进行分析。一般来说,它能以更快的速度实现更精确的化学成像。 结果:本文综述了RSI的最新进展,特别是仪器仪表的最新发展以及在生物医学和药物化学中的相关应用。最后,我们展望了肢体重复性劳损的发展趋势,以及它从实验室到临床的转化潜力。 结论:RSI技术是一种提供独特化学信息的强大技术,在生物医学和药物化学领域有着巨大的发展潜力。
关键词: 拉曼光谱成像,自发拉曼散射,点扫描,线扫描,宽视场成像,药物化学,生物医学应用
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