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Research Article

Gentianella turkestaum通过调节内质网应激和NF-κB信号通路显示对肝损伤的保护作用

卷 19, 期 6, 2019

页: [452 - 460] 页: 9

弟呕挨: 10.2174/1566524019666190415124838

价格: $65

摘要

目的：研究丁醇（指定为GBA）对Gentianella turkestaumum提取物对四氯化碳（CCl4）和过氧化氢（H2O2）诱导的肝细胞L02损伤的保护作用。 方法：将L02细胞与5μg/ mL，10μg/ mL，20μg/ mL，40μg/ mL，60μg/ mL，80μg/ mL和100μg/ mL GBA孵育24小时，然后进行MTT分析用于筛选GBA的细胞毒性。将细胞分为空白对照组，CCl4 / H2O2模型组，CCl4（20 mmol / L）或H2O2（100μmol/ L）处理;水飞蓟素+ CCl4 / H2O2组，CCl4（20 mmol / L）或H2O2（100μmol/ L）和5μg/ mL水飞蓟素处理; GBA + CCl4 / H2O2组，用CCl4（20mmol / L）或H2O2（100μmol/ L）和GBA（5μg/ mL，10μg/ mL和20μg/ mL）处理。进行MTT测定以确定细胞活性。使用商业试剂盒测定丙二醛（MDA）含量。测定上清液中的丙氨酸转氨酶（ALT），天冬氨酸转氨酶（AST）。采用PE-Annexin V / 7-ADD方法测定细胞凋亡。 RT-PCR用于评估内质网应激相关基因（CHOP，PERK，IRE1和ATF6）mRNA的表达。进行Western印迹分析以确定CHOP，Caspase 12和NF-κB蛋白的表达。 结果：GBA（5μg/ mL，10μg/ mL和20μg/ mL）孵育后的细胞存活率≥75％。 GBA孵育后，ALT和AST水平显着下降（P <0.05），而MDA水平显着下降（P <0.05）。与对照组相比，CCl4或H2O2组的细胞凋亡显着增加（P <0.05）。相比之下，与CCl4或H2O2组相比，GBA预孵育可以减弱细胞凋亡，其呈剂量依赖性方式（P <0.05）。在CCl4或H2O2存在下，CHOP，PERK，IRE1和ATF6 mRNA的表达显着上调（P <0.05）。然而，GBA此后诱导这些mRNA显着减少（P <0.05），同时CHOP和Caspase 12蛋白减少（P <0.05）。此外，它可以减弱核蛋白中NF-κBp65的表达。 结论：G。turkestanerum可抑制脂质过氧化，提高抗氧化活性。此外，它可以通过下调ERS相关基因和蛋白质的转录水平来抑制细胞凋亡。该过程与NF-κBp65蛋白的核转位相关。

关键词: Gentianella turkestanerum，肝脏L02细胞，肝损伤，四氯化碳，过氧化氢，丁醇。

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