Automated Acquisition of Aptamer Sequences

J. Colin Cox; Manjula Rajendran; Timothy Riedel; Eric A. Davidson; Letha J. Sooter; Travis S. Bayer; Mary Schmitz-Brown; Andrew D. Ellington

doi:10.2174/1386207023330291

Abstract

While the in vitro selection of nucleic acid binding species (aptamers) requires numerous liquidhandling steps, these steps are relatively straightforward and the overall process is therefore amenable to automation. Here we demonstrate that automated selection techniques are capable of generating aptamers against a number of diverse protein targets. Automated selection techniques can be integrated with automated analytical methods, including sequencing, determination of binding constants, and structural analysis. The methods that have so far been developed can be further multiplexed, and it should soon be possible to attempt the selection of aptamers against organismal proteomes or metabolomes.

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Combinatorial Chemistry & High Throughput Screening

Title: Automated Acquisition of Aptamer Sequences

Volume: 5 Issue: 4

Author(s): J. Colin Cox, Manjula Rajendran, Timothy Riedel, Eric A. Davidson, Letha J. Sooter, Travis S. Bayer, Mary Schmitz-Brown and Andrew D. Ellington

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Abstract: While the in vitro selection of nucleic acid binding species (aptamers) requires numerous liquidhandling steps, these steps are relatively straightforward and the overall process is therefore amenable to automation. Here we demonstrate that automated selection techniques are capable of generating aptamers against a number of diverse protein targets. Automated selection techniques can be integrated with automated analytical methods, including sequencing, determination of binding constants, and structural analysis. The methods that have so far been developed can be further multiplexed, and it should soon be possible to attempt the selection of aptamers against organismal proteomes or metabolomes.

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J. Colin Cox , Manjula Rajendran , Timothy Riedel , Eric A. Davidson , Letha J. Sooter , Travis S. Bayer , Mary Schmitz-Brown and Andrew D. Ellington , Automated Acquisition of Aptamer Sequences, Combinatorial Chemistry & High Throughput Screening 2002; 5 (4) . https://dx.doi.org/10.2174/1386207023330291