Occurrence of blaTEM and blaROB in Haemophilus species Causing Respiratory Tract Infections

Title:Occurrence of blaTEM and blaROB in Haemophilus species Causing Respiratory Tract Infections

Volume: 20 Issue: 3

Author(s): Prerna Khurana and Suchitra Shenoy*

Affiliation:

• Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka 576104,India

Keywords: Haemophilus, ROB, TEM, respiratory pathogen, sputum specimens, Haemophilus influenzae.

Abstract:

Background: Emergence of resistance to some antibiotics in Haemophilus influenzae, a respiratory pathogen is a cause of concern. The aim is to study the antibiotic susceptibility pattern of Haemophilus isolates from respiratory infections with reference to beta-lactam resistance.

Methods: This is a laboratory based prospective study done in the department of microbiology in a tertiary care center after institutional ethics committee clearance. Haemophilus influenzae isolates from respiratory tract specimens over a period of one year were subjected to antibiotic susceptibility tests. Beta-lactamase production was detected by nitrocefin disc. hpd gene, blaTEM and blaROB genes were detected by PCR. The data was analysed using SPSS 11.5 version.

Results: Of the 162 isolates, 89.5% were from sputum specimens. Ampicillin resistance was seen in 5 (3.09%) isolates. The ampicillin resistant strains were positive for beta-lactamase enzyme and blaTEM gene. BLNAR and isolates with blaROB gene were not found.

Conclusion: In case of Haemophilus influenzae respiratory tract infection empirical treatment with amoxicillin clavulanate or third generation cephalosporin may be the drugs of choice in our geographic area.

Cite this article as:

Khurana Prerna and Shenoy Suchitra *, Occurrence of blaTEM and blaROB in Haemophilus species Causing Respiratory Tract Infections, Infectious Disorders - Drug Targets 2020; 20 (3) . https://dx.doi.org/10.2174/1871526519666190118103148