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Infectious Disorders - Drug Targets

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ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

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Research Article

Characterization of Antibacterial-Producing Endophytic Fungi of Syzygiumpolyanthum Leaves

Author(s): Rahmita Burhamzah*, Gemini Alam and Herlina Rante

Volume 20, Issue 4, 2020

Page: [448 - 454] Pages: 7

DOI: 10.2174/1871526519666181226123541

Price: $65

Abstract

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers.

Aims and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves.

Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate.

Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm.

Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.

Keywords: Antibacterial, endophytic fungi, Syzygiumpolyanthum, antibiotic-resistant, immunosuppressant, host plants.

[1]
Baskaran, R.; Vijayakumar, R.; Mohan, P.M. Enrichment method for the isolation of bioactive actinomycetes from mangrove sediments of Andaman Islands, India. Malays. J. Microbiol., 2011, 7(1), 26-32.
[http://dx.doi.org/10.21161/mjm.24410]
[2]
Hardoim, Pablo Overbeek LS; van Bergr, Gabriele Pirttila; Anna Maria, Compat Matthias Seesitsch Angela The Hidden World within Plants: Ecological and Evolutionary Considerations for Defining Functioning of Microbial Endophytes. Microbiology and Molecular Biology Reviews., 2015, 79, 293-320.
[http://dx.doi.org/10.1128/MMBR. 00050-14.]
[3]
Strobel, G.; Daisy, B. Bioprospecting for microbial endophytes and their natural products. Microbiol. Mol. Biol. Rev., 2003, 67(4), 491-502.
[http://dx.doi.org/10.1128/MMBR.67.4.491-502.2003] [PMID: 14665674]
[4]
Tan, R.X.; Zou, W.X. Endophytes: a rich source of functional metabolites. Nat. Prod. Rep., 2001, 18(4), 448-459.
[http://dx.doi.org/10.1039/b100918o] [PMID: 11548053]
[5]
Kusuma, I.W.; Kuspradini, H.; Arung, E.T.; Aryani, F.; Min, Y.H.; Kim, J.S.; Kim, Y.U. Biological activity and phytochemical analysis of three Indonesian medicinal plants, Murraya koenigii, Syzygium polyanthum and Zingiber purpurea. J. Acupunct. Meridian Stud., 2011, 4(1), 75-79.
[http://dx.doi.org/10.1016/S2005-2901(11)60010-1] [PMID: 21440883]
[6]
Bester, C. Investigating the Efficacy of Sodium Hypochlorite, Calcium Hypochlorite and Peracetic Acid on Environmental Escherichia coli Strains. Thesis of Master Degree. University of Stellenbosch. Stellenbosch, Western Cape, South Africa. December. 2015.
[7]
Desale, M. Antimicrobial Activity of Endophytic Fungi Isolat-ed from Ocimum sanctum Linn. American International Journal of Contemporary Scientific Research., 2014, 1, 17-22.
[8]
Arora, D.S. And Kaur, N. 2018. Antimicrobial Potential of Fungal Endophytes from Moringa oleifera. Appl. Biochem. Biotechnol., 2018.
[http://dx.doi.org/10.1007/s12010-018-2770-y] [PMID: 30039472]
[9]
Xu, J. Fungal DNA barcoding. Genome, 2016, 59(11), 913-932.
[http://dx.doi.org/10.1139/gen-2016-0046] [PMID: 27829306]
[10]
Jie, W.; Ling-yun, Y.; Lu, Y. Ceriporialacerata DMC1106, a New Endophytic Fungus: Isolation, Identification, and Opti-mal Medium for 2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone Production. Biotechnol. Bioprocess Eng.; BBE, 2013, 18, 669-678.
[http://dx.doi.org/10.1007/s12257-012-0846-z]
[11]
Sardul, S.S.; Suneel, K.; Ravindra, P.A. Isolation and identifi-cation of endophytic fungi from RicinuscommunisLinn. And their antibacterial activity. IJRPC, 2014, 4(3), 611-618.
[12]
Powthong, P.; Jantrapanukorn, B.; Thongmee, A.; Sun-tornthiticharoen, P. Screening of antimicrobial activities of the endophytic fungi isolated from Sesbania grandiflora (L.). Pers. J. Agr. Sci. Tech., 2013, 15, 1513-1522.
[13]
Balouiri, M.; Sadiki, M.; Ibnsouda, S.K. Methods for in vitro evaluating antimicrobial activity: A review. J. Pharm. Anal., 2016, 6(2), 71-79.
[http://dx.doi.org/10.1016/j.jpha.2015.11.005] [PMID: 29403965]
[14]
Genomic DNA Extraction Kit Protocol Book. http://www.geneaid.com/products/reagent-genomic-dna-extraction/dna-extraction-kit-yeast-fungus-gentra-puregene-alternative

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