Abstract
Background: Within the healthcare industry, including the care of chronic wounds, the challenge of antimicrobial resistance continues to grow. As such, there is a need to develop new treatments that can reduce the bioburden in wounds.
Objective: The present study is focused on the development of polyaniline (PANI) / chitosan (CH) nanofibrous electrospun membranes and evaluates their antibacterial properties.
Methods: To this end, experimental design was used to determine the electrospinning windows of both pure chitosan and PANI/CH blends of different ratios (1:3, 3:5, 1:1). The effect of key environmental and process parameters (relative humidity and applied voltage) was determined, as well as the effect of the PANI/CH ratio in the blend and the molecular interactions between PANI and chitosan that led to jet stability.
Results: The nanofibrous mats were evaluated regarding their morphology and antibacterial effect against model gram positive and gram negative bacterial strains, namely B. subtilis and E. coli. High PANI content mats show increased bactericidal activity against both bacterial strains.
Conclusion: The blend fibre membranes combine the materials’ respective properties, namely electrical conductivity, biocompatibility and antibacterial activity. This study suggests that electrospun PANI/CH membranes are promising candidates for healthcare applications, such as wound dressings.
Keywords: Polyaniline, chitosan, nanofibers, wound dressing, antimicrobial properties, electrospinning parameters, voltage, humidity.
Graphical Abstract
[1]
Punjabi PB, Chauhan NPS, Jangid NK, Juneja P. Conducting polymers: biodegradable tissue engineering. Encycl Biomed Polym Polym Biomater 2015; 11: 1972-81.
[2]
Katti DS, Robinson KW, Ko FK, Laurencin CT. Bioresorbable nanofiber-based systems for wound healing and drug delivery: optimization of fabrication parameters. J Biomed Mater Res 2004; 70B(2): 286-96.
[3]
Cargill M, Ireland JS, Bolk S, Roxbury W, Us MA, Mccarthy JJ. Dispensing device and method for forming material United States Patent US 6,252,129 B 2001 Jun 26..
[4]
Moutsatsou P, Coopman K, Georgiadou S. Biocompatibility assessment of conducting PANI/chitosan nanofibers for wound healing applications. Polymers 2017; 9(12): 687.
[5]
Abrigo M, McArthur SL, Kingshott P. Electrospun nanofibers as dressings for chronic wound care: advances, challenges, and future prospects. Macromol Biosci 2014; 14(6): 772-92.
[6]
Huang Z, Zhang Y, Kotaki M, Ramakrishna S. A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol 2003; 63: 2223-53.
[7]
Gu BK, Park SJ, Kim MS, Kang CM, Kim J. Il, Kim CH. Fabrication of sonicated chitosan nanofiber mat with enlarged porosity for use as hemostatic materials. Carbohydr Polym 2013; 97(1): 65-73.
[8]
Croisier F, Jérôme C. Chitosan-based biomaterials for tissue engineering. Eur Polym J 2013; 49(4): 780-92.
[9]
Marcasuzaa P, Reynaud S, Ehrenfeld F, Khoukh A, Desbrieres J. Chitosan-graft-polyaniline-based hydrogels: elaboration and properties. Biomacromolecules 2010; 11(6): 1684-91.
[10]
Jayakumar R, Prabaharan M, Reis RL, Mano JF. Graft copolymerized chitosan - present status and applications. Carbohydr Polym 2005; 62(2): 142-58.
[11]
Rabea EI, Badawy MET, Stevens CV, Smagghe G, Steurbaut W. Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules 2003; 4(6): 1457-65.
[12]
El Hadrami A, Adam LR, El Hadrami I, Daayf F. Chitosan in plant protection. Mar Drugs 2010; 8(4): 968-87.
[13]
Helander IM, Nurmiaho-Lassila EL, Ahvenainen R, Rhoades J, Roller S. Chitosan disrupts the barrier properties of the outer membrane of Gram-negative bacteria. Int J Food Microbiol 2001; 71(2–3): 235-44.
[14]
Shiy N, Guo X, Hemin Jing, Gong Yang J, Sun C. Antibacterial effect of the conducting polyaniline. J Mater Sci Technol 2006; 22(3): 289-90.
[15]
Kucekova Z, Kasparkova V, Humpolicek P, Sevcikova P, Stejskal J. Antibacterial properties of polyaniline-silver films. Chem Pap 2013; 67(8): 1103-8.
[16]
Gizdavic-Nikolaidis MR, Bennett JR, Swift S, Easteal AJ, Ambrose M. Broad spectrum antimicrobial activity of functionalized polyanilines. Acta Biomater 2011; 7(12): 4204-9.
[17]
Humpolicek P, Kasparkova V, Saha P, Stejskal J. Biocompatibility of polyaniline. Synth Met 2012; 162(7–8): 722-7.
[18]
Ohkawa K, Cha D, Kim H, Nishida A, Yamamoto H. Electrospinning of chitosan. Macromol Rapid Commun 2004; 25(18): 1600-5.
[19]
Sun K, Li ZH. Preparations, properties and applications of chitosan based nanofibers fabricated by electrospinning. Express Polym Lett 2011; 5(4): 342-61.
[20]
Casasola R, Thomas NL, Georgiadou S. Electrospinning of poly(lactic acid): theoretical approach for the solvent selection to produce defect-free nanofibers. J Polym SciPart B, Polym Phys 2016; 54(15): 1483-98.
[21]
Nizioł J, Gondek E, Plucinski KJ. Characterization of solution and solid state properties of polyaniline processed from trifluoroacetic acid. J Mater Sci Mater Electron 2012; 23(12): 2194-201.
[23]
Torres-Giner S, Ocio MJ, Lagaron JM. Development of active antimicrobial fiber based chitosan polysaccharide nanostructures using electrospinning. Eng Life Sci 2008; 8(3): 303-14.
[24]
Frohbergh ME, Katsman A, Botta GP, et al. Electrospun hydroxyapatite-containing chitosan nanofibers crosslinked with genipin for bone tissue engineering. Biomaterials 2012; 33(36): 9167-78.
[25]
Ma X, Ge J, Li Y, Guo B, Ma PX. Nanofibrous electroactive scaffolds from a chitosan-grafted-aniline tetramer by electrospinning for tissue engineering. RSC Adv 2014; 4(26): 13652-61.
[26]
Dhivya C, Vandarkuzhali SAA, Radha N. Antimicrobial activities of nanostructured polyanilines doped with aromatic nitro compounds. Arab J Chem 2015; 8.
[27]
Sill TJ, Von-Recum HA. Electrospinning: applications in drug delivery and tissue engineering. Biomaterials 2008; 29(13): 1989-2006.
[28]
Pelipenko J, Kristl J, Janković B, Baumgartner S, Kocbek P. The impact of relative humidity during electrospinning on the morphology and mechanical properties of nanofibers. Int J Pharm 2013; 456(1): 125-34.
[29]
De-Vrieze S, Van-Camp T, Nelvig A, Hagström B, Westbroek P, De Clerck K. The effect of temperature and humidity on electrospinning. J Mater Sci 2009; 44(5): 1357-62.
[30]
Li W, Shanti RM, Tuan RS. Electrospinning technology for nanofibrous scaffolds in tissue engineering.CSSR K, editor Nanotechnologies Life Sci. 2006; 9: pp. (October 2015)135-87.
[31]
Zhang Y. Electrospun nanofibers with tunable electrical conductivity Available at: 2013.http://dspace.mit.edu/ handle/1721.1/81690
[32]
Moutsatsou P, Coopman K, Smith MB, Georgiadou S. Conductive PANI fibers and determining factors for the electrospinning window. Polymer 2015; 77: 143-51.
[33]
Zhang C, Yuan X. Study on morphology of electrospun poly (vinyl alcohol) mats. Eur Polym J 2005; 41: 423-32.
[34]
Meechaisue C, Dubin R, Supaphol P, Hoven VP, Kohn J. Electrospun mat of tyrosine-derived polycarbonate fibers for potential use as tissue scaffolding material. In: J Biomater Sci Polym Ed. 2006; 17: pp. (9)039-56.
[35]
Terada D, Kobayashi H, Zhang K, Tiwari A, Yoshikawa C, Hanagata N. Transient charge-masking effect of applied voltage on electrospinning of pure chitosan nanofibers from aqueous solutions. Sci Technol Adv Mater 2012; 13(1): 15003.
[36]
Foreman JP, Monkman AP. Theoretical investigations into the structural and electronic influences on the hydrogen bonding in doped polyaniline. Synth Met 2003; 107(38): 135-6.
[37]
Łuzny W, Piwowarczyk K. Hydrogen bonds in camphorsulfonic acid doped polyaniline. Polimery 2011; 56(9): 652-6.
[38]
Ma G, Liu Y, Fang D, et al. Hyaluronic acid/chitosan polyelectrolyte complexes nanofibers prepared by electrospinning. Mater Lett 2012; 74: 78-80.
[39]
Ishihara M, Kosaka T, Nakamura T, et al. Antibacterial activity of fluorine incorporated DLC films. Diam Relat Mater 2006; 15(4-8): 1011-4.
[40]
Goy RC, Morais STB, Assis OBG. Evaluation of the antimicrobial activity of chitosan and its quaternized derivative on E. Coli and S. aureus growth. Rev Bras Farmacogn 2016; 26: 122-7.
[41]
Salton MRJ, Kim K-S. Chapter 2: Structure..In: Baron S, editor Medical Microbiology 4th Editio. Galveston, TX: University of Texas Medical Branch at Galveston 1996.
[42]
Karami Z, Rezaeian I, Zahedi P, Abdollahi M. Preparation and performance evaluations of electrospun poly(ε- caprolactone), poly(lactic acid), and their hybrid (50/50) nanofibrous mats containing thymol as an herbal drug for effective wound healing. J Appl Polym Sci 2013; 129(2): 756-66.
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