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Current Traditional Medicine

Editor-in-Chief

ISSN (Print): 2215-0838
ISSN (Online): 2215-0846

Research Article

Effect of Geographical and Seasonal Variations on Phenolic Contents and Antioxidant Activity of Aerial Parts of Urtica diocia L.

Author(s): Vinod Kumar Gautam, Mannu Datta and Ashish Baldi*

Volume 5, Issue 2, 2019

Page: [159 - 167] Pages: 9

DOI: 10.2174/2215083804666181012123333

Price: $65

Abstract

Background: Environmental conditions affect the biosynthesis of secondary metabolites as a result of biotic and abiotic factors. In the present study, the effect of different geographical locations and season was studied on total phenolic and flavonoid contents extracted from Urtica dioica Linn.

Methodology: The aerial parts of U. dioica collected from Palampur, Shimla and Dharamshala in different seasons were subjected to hydro-alcoholic extraction. Quantitative estimation of total phenolic and flavonoid contents in various extracts was carried out spectrophotometrically.

Results: The highest amount of total phenolic (3.987± 0.130) and flavonoid contents (2.847± 0.341) was found in Palampur sample collected in summer season whereas sample collected from Dharamshala in spring season showed the least phenolic contents. In vitro antioxidant activity of all the samples was evaluated by DPPH, NO scavenging and FRPA method. The antioxidant potential was found maximum in the sample collected from Palampur in the summer season, however, the sample collected from Dharamshala in spring season showed the least antioxidant potential.

Conclusion: The present study confirms that altitude and seasonal variations significantly affect the levels of secondary metabolites in plant parts.

Keywords: Antioxidant, flavonoids, polyphenols, seasonal variation, Urtica dioica Linn, secondary metabolites.

Graphical Abstract

[1]
Tamilselvan N, Thirumalai T, Elumalai EK, Balji E, David E. Pharmacognosy of coccinia grandis. Asian Pac J Trop Biomed 2013; 3: 299-300.
[2]
Patel VR, Patel PR, Kajal SS. Antioxidant activity of some selected medicinal plant in western region of India. Adv Bio Res 2010; 4: 23-6.
[3]
Soni U, Brar S, Gauttam VK. Effect of seasonal variation on secondary metabolites of medicinal plants. Int J Pharm Sci Res 2015; 6(9): 3654.
[4]
Niazi J, Kaur N, Gupta V. Coccinia indica; A boon from tropics. Int J Adv Pharm Sce 2013; 4: 637-46.
[5]
Shohael AM, Ali MB, Yu KW, Hahn EJ. Effect of temperature on secondary metabolites production and antioxidant enzyme activities in eleutherococcus senticosus somatic embryos. Plant Cell Tissue Organ Cult 2006; 85(2): 219-22.
[6]
Gairola S, Shariff NM, Batt A, Kala CP. Influence of climate change on production of secondary chemicals in high altitude medicinal plants: Issues needs immediate attention. J Med Plants Res 2010; 1825-9.
[7]
Rahman H, Eswaraiah MC, Dutta AM. In-vitro anti-oxidant activity of citrus macroptera (var annamensis) Fruit peels extracts. Int J Pharm Pharm Sci 2014; 6: 1-7.
[8]
Joshi BC, Mukhija M, Kalia AN. Pharmacognostical review of Urtica dioica. L. Int J Green Pharm 2014; pp. 201-6.
[9]
Joshi BC, Prakash A, Kalia AN. Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl4 challenged rats. Toxicol Rep 2015; 1101-10.
[10]
Bisset NG. Herbal drugs and phytopharmaceuticals Boca Raton. CRC Press 1994; p. 105.
[11]
Bharti KA, Sharma B. Plants used as folk medicine in sikkim Himalayas. Ethnobot Res Appl 2012; 10: 339-56.
[12]
Kawy A, Moatafa A. Quality control of certain slimming herbal products present in the egyptian market. Life Sci J 2012; 9: 3-10.
[13]
Joshi BC, Mukhija M, Sushmita S. Antioxidant potential and total phenolic content of urtica dioica (whole plant). J Appl Pharm Sci 2015; 7(2): 201-6.
[14]
Maurya S, Singh D. Quantitative analysis of total phenolic content in adhatoda vasica nees extracts. Int J Pharm Tech Res 2010; 2(4): 2403-6.
[15]
Mohammed HA, Salmin KA, Awad GA. Antioxidant and quantitative estimation of phenolic and flavonoids of three halophytic plants growing in Libya. J Pharmacog Phytochem 2013; 2(3): 89-94.
[16]
Sultana M, Verma PK, Raina R, Dar MA. Quantitative analysis of total phenolic, flavonoids and tannin contents in acetone and n-hexane extracts of ageratum conyzoides. Int J Chemtech Res 2012; 4(3): 996-9.
[17]
Choi C, Kim WS, Hwang SS. Antioxidant activity and free radical scavenging capacity between Korean medicinal plants and flavonoids by assay-guided comparison. Plant Sci 2002; 163(6): 1161-8.
[18]
Thakral J, Kalia AN. Antioxidant potential fractionation from methanol extract of aerial parts of convolvulus arvensis Linn. (convolvulaceae). Int J Pharm Sci Drug Res 2010; 2(3): 219-23.
[19]
Nagulendran KR, Velavan S, Mahesh R, Hazeena V. In-vitro antioxidant activity and total polyphenolic content of cyperus rotundus rhizomes. Eur J Chem 2007; 4: 440-9.
[20]
Michael JS, Kalirajan A, Singh AJ, Padmalatha C. In vitro antioxidant evaluation and total phenolics of methanolic leaf extracts of nyctanthes arbortristis L. Chin J Nat Med 2013; 11(5): 484-7.
[21]
Maurya S, Singh D. Quantitative analysis of total phenolic content in adhatoda vasica nees extracts. Int J Pharm Tech Res 2010; 2(4): 2403-6.
[22]
Akula R, Gokare AR. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav 2011; 6(11): 1720-31.
[23]
Gouvea D, Leonardo RO, Norberto LP. The influence of biotic and abiotic factors on the production of secondary metabolites in medicinal plants. Plant Bioactives and Drug Discovery: Principles, Practice, and Perspectives 2012; 17: 419.
[24]
Gairola S, Shariff NM, Batt A, Kala CP. Influence of climate change on production of secondary chemicals in high altitude medicinal plants: Issues needs immediate attention. Med J Plants Res 2010; pp. 1825-9.
[25]
Salik J, Fangb Z, Byg A. Eastern himalayan alpine plant ecology, Tibetan ethnobotany, and climate change. Glob Environ Change 2009; 19: 147-55.
[26]
Choudhry N, Singh S, Siddhiqui MB, Khatoon S. Impact of seasons and dioecy on therapeutic phytoconstituents of tinospora cordifolia, a rasayana drug. BioMed Res Int 2014; 6: 64-72.
[27]
Dildar A, Baig H, Zara S. Seasonal variation of phenolics, flavonoids, antioxidant and lipid peroxidation inhibitory activity of methanolic extract of melilotus indicus and its sub-fractions in different solvents. Int J Phytomed 2012; 326-32.
[28]
Wahid A. Heat tolerance in plants: An overview. Environ Exp Bot 2007; 61(3): 199-223.
[29]
Rejeb IB, Victoria P, Brigitte MM. Plant responses to simultaneous biotic and abiotic stress: Molecular mechanisms. Plants 2014; 3(4): 458-75.
[30]
Baldi A, Hussain W, Tailor Y. Evaluation of in-vitro cultured cells of withania somnifera for antioxidant activity. Curr Trend in Biotech and Pharm 2010; 4: 589-95.
[31]
Baldi A, Choudhary N. In vitro antioxidant and hepatoprotective potential of chenopodium album extract. Int J Green Pharm 2013; 7(1): 50-6.
[32]
Baldi A. Evaluation of in vitro antioxidant activity of eclipta alba, Acta Hort 972 Int Soc Hortic Sci 2013; 27-30.
[33]
Baldi A, Pandit MK, Ranka P. Amelioration of in-vivo antioxidant activity by banana extracts. Int J Pharm Biol Arch 2012; 3(1): 157-61.
[34]
Roy A, Saraf S. Antioxidant and antiulcer activities of an ethnomedicine: Alternanthera sessilis. Res J Pharm Technol 2008; 1: 75-9.
[35]
Singh PA, Brindavanam NB, Kimothi GP, Aeri V. Evaluation of in vivo anti-inflammatory and analgesic activity of dillenia indica f. elongata Miq. and shorea robusta stem bark extracts. Asian Pac J Trop Dis 2016; 6(1): 75-81.
[36]
Singh PA, Brindavanam NB, Kimothi GP, Verma R, Aeri V. A validated HPLC method for the determination of betulin in the stem bark of tectona grandis Linn. Int J Pharm Sci Res 2016; 7(2): 719-23.
[37]
Singh PA, Vidhu A. Physico-chemical parameters and HPTLC fingerprinting profile of dillenia indica Miq. f. elongata (Miq.) and tectona grandis Linn. with reference to betulin. Int J Pharma Bio Sci 2016; 7(1): 217-21.
[38]
Harbowy ME, Balentine DA. Tea chemistry. Crit Rev Plant Sci 1997; 16: 415-80.
[39]
Kolawole OT, Ayankunle AA. Seasonal variation in the anti-diabetic and hypolipidemic effects of momordica charantia fruit extract in rats. European J Med Plants 2012; 2(2): 177.
[40]
Sandeep RP, Mansingraj SN, Nilesh VP, Rajaram PP, Ghansham BD. Seasonal discrepancy in phenolic content and antioxidant properties from bark of nothapodytes nimmoniana. Int J Pharma Bio Sci 2010; 1(3): 1-17.
[41]
Sati P, Pandey A, Rawat S, Rani A. Phytochemicals and antioxidants in leaf extracts of ginkgo biloba with reference to location, seasonal variation and solvent system. J Pharm Res 2013; 7(9): 804-9.
[42]
Soni U, Brar S, Gauttam VK. Effect of seasonal variation on secondary metabolites of medicinal plants. Int J Pharm Sci Res 2015; 6(9): 3654-62.

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