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Recent Patents on Food, Nutrition & Agriculture

Editor-in-Chief

ISSN (Print): 2212-7984
ISSN (Online): 1876-1429

Research Article

Maximizing Biomass and Lipid Production in Heterotrophic Culture of Chlorella vulgaris: Techno-Economic Assessment

Author(s): Mohammad H. Morowvat* and Younes Ghasemi

Volume 10, Issue 2, 2019

Page: [115 - 123] Pages: 9

DOI: 10.2174/2212798410666180911100034

Abstract

Background: Nowadays, chlorophycean microalgae have attained a broad-spectrum attention as a potential candidate for biomass and bioenergy production. Despite their appreciated benefits, one of major problems is their low biomass and lipid productivity. Here we investigated the heterotrophic culture in shake flasks and stirred tank bioreactor to improve the lipid and biomass production in a naturally isolated strain of Chlorella vulgaris.

Methods: A naturally isolated C. vulgaris strain was cultivated in BG-11 medium in shake flask and bioreactor. Its biochemical composition and growth kinetic parameters were investigated.

Results: The biomass productivity was improved (3.68 fold) under heterotrophic culture compared to basal autotrophic culture condition in shake flask experiment. The total lipid content increased to 44% of total Dry Cell Weight (DCW) during heterotrophic growth after 21 days. Moreover, a great Fatty Acid Methyl Esters (FAME) yield was observed under heterotrophic cultivation. Total biomass and lipid content of microalgae in bioreactor experiment increased to 4.95 and 2.18 g L-1 respectively, during 5 days of the experiment compared to its basic autotrophic culture.

Conclusion: The techno-economic aspects of exploiting C. vulgaris as a biodiesel feedstock werealso evaluated. The results imply that heterotrophic cultivation could compensate the low biomass productivity in microalgae for green energy production. Ever growing rates of established patents on application of various genetic and bioengineering-based methods have made it possible to achieve higher lipid contents with reduced total costs for microalgal biodiesel production as well.

Keywords: Biomass, Chlorella vulgaris, heterotrophic culture, lipid, techno-economic assessment, food supplements.

Graphical Abstract

[1]
Spolaore P, Joannis-Cassan C, Duran E, Isambert A. Commercial applications of microalgae. J Biosci Bioeng 2006; 101(2): 87-96.
[2]
Bennion EP, Ginosar DM, Moses J, Agblevor F, Quinn JC. Lifecycle assessment of microalgae to biofuel: Comparison of thermochemical processing pathways. Appl Energy 2015; 154: 1062-71.
[3]
Fargione J, Hill J, Tilman D, Polasky S, Hawthorne P. Land clearing and the biofuel carbon debt. Science 2008; 319(5867): 1235-8.
[4]
Lapinskienė A, Martinkus P, Rėbždaitė V. Eco-toxicological studies of diesel and biodiesel fuels in aerated soil. Environ Pollut 2006; 142(3): 432-7.
[5]
Lapuerta M, Armas O, Rodríguez-Fernández J. Effect of biodiesel fuels on diesel engine emissions. Pror Energy Combust Sci 2008; 34(2): 198-223.
[6]
Razon LF, Tan RR. Net energy analysis of the production of biodiesel and biogas from the microalgae: Haematococcus pluvialis and Nannochloropsis. Appl Energy 2011; 88(10): 3507-14.
[7]
Yang C, Hua Q, Shimizu K. Energetics and carbon metabolism during growth of microalgal cells under photoautotrophic, mixotrophic and cyclic light-autotrophic/dark-heterotrophic conditions. Biochem Eng J 2000; 6(2): 87-102.
[8]
Suh I, Lee C-G. Photobioreactor engineering: Design and performance. Biotechnol Bioproc Eng 2003; 8(6): 313-21.
[9]
Grobbelaar J. Microalgae mass culture: the constraints of scaling-up. J Appl Phycol 2012; 24(3): 315-8.
[10]
Perez-Garcia O, Escalante FME, de-Bashan LE, Bashan Y. Heterotrophic cultures of microalgae: Metabolism and potential products. Water Res 2011; 45(1): 11-36.
[11]
Behrens PW. Photobioreactor and fermentors: The light and the dark sides of the growing algae. In: Andersen RA, Ed. Algal Culturing Techniques. New York, USA: Elsevier Academic Press 2005; pp. 189-204.
[12]
Eriksen N. The technology of microalgal culturing. Biotechnol Lett 2008; 30(9): 1525-36.
[13]
Amaro HM, Guedes AC, Malcata FX. Advances and perspectives in using microalgae to produce biodiesel. Appl Energy 2011; 88(10): 3402-10.
[14]
Davis R, Aden A, Pienkos PT. Techno-economic analysis of autotrophic microalgae for fuel production. Appl Energy 2011; 88(10): 3524-31.
[15]
Xu H, Miao X, Wu Q. High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. J Biotechnol 2006; 126(4): 499-507.
[16]
Liu J, Huang J, Sun Z, Zhong Y, Jiang Y, Chen F. Differential lipid and fatty acid profiles of photoautotrophic and heterotrophic Chlorella zofingiensis: Assessment of algal oils for biodiesel production. Bioresour Technol 2011; 102(1): 106-10.
[17]
Cheirsilp B, Torpee S. Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation. Bioresour Technol 2012; 110: 510-6.
[18]
Morowvat MH, Babaeipour V, Rajabi-Memari H, Vahidi H, Maghsoudi N. Overexpression of recombinant human beta interferon (rhINF-β) in periplasmic space of Escherichia coli. Iran J Pharm Res 2014; 13(Suppl.): 151-60.
[19]
Morowvat MH, Ghasemi Y. Culture medium optimization for enhanced β-carotene and biomass production by Dunaliella salina in mixotrophic culture. Biocatal Agric Biotechnol 2016; 7: 217-23.
[20]
Hu Q, Xiang W, Dai S, Li T, Yang F, Jia Q, et al. The influence of cultivation period on growth and biodiesel properties of microalga Nannochloropsis gaditana 1049. Bioresour Technol 2015; 192: 157-64.
[21]
Fei Q, Chang HN, Shang L, Choi J-D-R, Kim N, Kang J. The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production. Bioresour Technol 2011; 102(3): 2695-701.
[22]
Fei Q, Fu R, Shang L, Brigham CJ, Chang HN. Lipid production by microalgae Chlorella protothecoides with volatile fatty acids (VFAs) as carbon sources in heterotrophic cultivation and its economic assessment. Bioprocess Biosyst Eng 2015; 38(4): 691-700.
[23]
Hempel N, Petrick I, Behrendt F. Biomass productivity and productivity of fatty acids and amino acids of microalgae strains as key characteristics of suitability for biodiesel production. J Appl Phycol 2012; 24(6): 1407-18.
[24]
Griffiths MJ, Harrison STL. Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 2009; 21(5): 493-507.
[25]
Rodolfi L, Chini G, Bassi N, Padovani G, Biondi N, Bonini G, et al. Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 2008; 102.
[26]
Magri M. A new microalgae Chlorella for production of vegetal oil for biodiesel and cogeneration power units patent. EP3031932 . 2016.
[27]
Gopinath A, Puhan S, Nagarajan G. Effect of biodiesel structural configuration on its ignition quality. Int J Energy Environ 2010; 1(2): 295-306.
[28]
Atabani AE, César ADS. Calophyllum inophyllum L. - A prospective non-edible biodiesel feedstock. Study of biodiesel production, properties, fatty acid composition, blending and engine performance. Renew Sustain Energy Rev 2014; 37: 644-55.
[29]
Sorate KA, Bhale PV. Biodiesel properties and automotive system compatibility issues. Renew Sustain Energy Rev 2015; 41: 777-98.
[30]
Monirul IM, Masjuki HH, Kalam MA, Zulkifli NWM, Rashedul HK, Rashed MM, et al. A comprehensive review on biodiesel cold flow properties and oxidation stability along with their improvement processes. RSC Advances 2015; 5(105): 86631-55.
[31]
Franklin S, Brubaker S, Rudenko GN, Bhat R, Shoa-azar M, Somanchi A, et al. Solazyme, Inc. Genetically engineered microbial strains including Chlorella protothecoides lipid pathway genes. US20140178950 . 2014.
[32]
Materials ASfT, editor ASTM D6751-15ce1, Standard specification for biodiesel fuel blend stock (B100) for middle distillate fuels In: 2015.ASTM International, West Conshohocken, PA..
[33]
Zheng Y, Li T, Yu X, Bates PD, Dong T, Chen S. High-density fed-batch culture of a thermotolerant microalga Chlorella sorokiniana for biofuel production. Appl Energy 2013; 108: 281-7.
[34]
Mu J, Li S, Chen D, Xu H, Han F, Feng B, et al. Enhanced biomass and oil production from sugarcane bagasse hydrolysate (SBH) by heterotrophic oleaginous microalga Chlorella protothecoides. Bioresour Technol 2015; 185(Suppl. C): 99-105.
[35]
Lin W, Luo J, Xie Z. Mixotrophic cultivation method of Chlorella by using xylose. CN106479895 . 2017.
[36]
Shen X-F, Liu J-J, Chu F-F, Lam PKS, Zeng RJ. Enhancement of FAME productivity of Scenedesmus obliquus by combining nitrogen deficiency with sufficient phosphorus supply in heterotrophic cultivation. Appl Energy 2015; 158: 348-54.
[37]
Morales-Sánchez D, Tinoco-Valencia R, Kyndt J, Martinez A. Heterotrophic growth of Neochloris oleoabundans using glucose as a carbon source. Biotechnol Biofuels 2013; 6(1): 1-13.
[38]
Lu J, Sheahan C, Fu P. Metabolic engineering of algae for fourth generation biofuels production. Energy Environ Sci 2011; 4(7): 2451-66.

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