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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

Dynamic Changes of Eight Bioactive Constituents in Magnoliae Officinalis Cortex Based on UFLC-QTRAP-MS/MS Combined with Grey Relational Analysis

Author(s): Hui Zhao, Ying Yan, Chuan Chai, Li-Si Zou, Xun-hong Liu*, Sheng-Nan Wang and Yu-Jiao Hua

Volume 15, Issue 5, 2019

Page: [497 - 504] Pages: 8

DOI: 10.2174/1573412914666180903123749

Price: $65

Abstract

Background: Magnoliae Officinalis Cortex is a well-known traditional Chinese herbal medicine with vast clinical consumption owing to its positive effects. However, little attention has been devoted to analyzing the dynamic changes of the chemical constituents of Magnoliae Officinalis Cortex in different growth periods.

Methods: In this study, all analyses were performed on UFLC -20ADXR system and a SynergiTM Hydro-RP 100 Å column (100 mm×2.0 mm, 2.5µm). The mobile phase consisted of water containing 0.1% formic acid (A) and acetonitrile containing 0.1% formic acid (B). The target constituents, including two lignans, two alkaloids, two flavonoids, one phenylpropanoid glycoside, and one organic acid, were analyzed in both positive and negative ion modes with accurate and sensitive multiple reaction monitoring (MRM) mode.

Results: The correlation coefficients of all the calibration curves were higher than 0.9992. Relative standard deviations of intra- and inter-day precisions of the eight analytes were all lower than 4.01% and the recoveries were in the range from 98.62% to 102.46%. Grey relational analysis was performed to evaluate the samples according to the contents of 8 constituents. The results showed that the quality of Magnoliae Officinalis Cortex collected at traditional harvest time was much better, and the higher the age, the better the quality.

Conclusion: The proposed method is useful for the assessment on the quality of Magnoliae Officinalis Cortex, and this study provides the basis for exploring the quality forming mechanism of Magnoliae Officinalis Cortex medicinal materials and choosing the suitable harvesting period.

Keywords: Magnoliae officinalis cortex, dynamic accumulation, UFLC-QTRAP-MS/MS, simultaneous quantitation, GRA, bioactive.

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[1]
Committee, N.P. Pharmacopoeia of The People’s Republic of China. China Medical Science Press: Beijing,, 2015. Vo1.1, p 251- 252.
[2]
Fu, Y.H.; Liu, B.; Zhang, N.S.; Liu, Z.C.; Liang, D.J.; Li, F.Y.; Cao, Y.G.; Fenf, X.S.; Zhang, X.C.; Yang, Z.T. Magnolol inhibits lipopolysaccharide-induced inflammatory response by interfering with TLR4 mediated NF-kappaB and MAPKs signaling pathways. J. Ethnopharmacol., 2013, 145(1), 193-199.
[3]
Li, C.Y.; Chao, L.K.; Wang, S.C.; Chang, H.Z.; Tsai, M.L.; Fang, S.H. Honokiol inhibits LPS-induced maturation and inflammatory response of human monocyte-derived dendritic cells. J. Cell. Physiol., 2011, 226(9), 2338-2349.
[4]
Zhang, H.M.; Ren, H.; He, Y.; Liu, F. Effects of Magnolia Officinalis with different extraction techniques on analgesia function in mice. Nat. Sci. J. Chengdu Univ., 2013, 32(3), 235-237.
[5]
Shen, J.L.; Man, K.M.; Huang, P.H.; Chen, W.C.; Chen, D.C.; Cheng, Y.W.; Liu, P.L.; Chou, M.C.; Chen, Y.H. Honokiol and magnolol as multifunctional antioxidative molecules for dermatologic disorders. Molecules, 2010, 15(9), 6452-6465.
[6]
Chilampalli, C.; Zhang, X.; Kaushik, R.S.; Young, A.; Zeman, D.; Hildreth, M.B.; Fahmy, H.; Dwivedi, C. Chemopreventive effects of combination of honokiol and magnolol with alpha-santalol on skin cancer developments. Drug Discov. Ther., 2013, 7(3), 109-115.
[7]
Kumar, A.; Kumar Singh, U.; Chaudhary, A. Honokiol analogs: A novel class of anticancer agents targeting cell signaling pathways and other bioactivities. Future Med. Chem., 2013, 5(7), 809-829.
[8]
Arora, S.; Bhardwaj, A.; Srivastava, S.K.; Singh, S.; McClellan, S.; Wang, B.; Singh, A.P. Honokiol arrests cell cycle, induces apoptosis, and potentiates the cytotoxic effect of gemcitabine in human pancreatic cancer cells. PLoS One, 2011, 6(6), e21573.
[9]
Luo, L.; Nong-Wang, J.; Kong, L.D.; Jiang, Q.G.; Tan, R.X. Antidepressant effects of BanxiaHoupu decoction, a traditional Chinese medicinal empirical formula. J. Ethnopharmacol., 2000, 73(1), 277-281.
[10]
Pang, Y.L.; Han, X.F.; Bamikole, M.A.; Deng, Y.L. Anti-diarrhea and anti-oxidant properties of Magnolol. Trop. J. Pharm. Res., 2013, 12(1), 85-91.
[11]
Li, J.M.; Kong, L.D.; Wang, Y.M.; Cheng, C.H.; Zhang, W.Y.; Tan, W.Z. Behavioral and biochemical studies on chronic mild stress models in rats treated with a Chinese traditional prescription Banxia-houpu decoction. Life Sci., 2003, 74(1), 55-73.
[12]
Sumino, M.; Saito, Y.; Ikegami, F.; Hirasaki, Y.; Namiki, T. Efficient preparation of Hangekobokuto (Banxia-Houpo-Tang) decoction by adding perilla herb before decoction is finished. Nat. Prod. Commun., 2011, 7(12), 1619-1622.
[13]
Shen, F.Y.; Wei, H.Z.; Sun, Y.B.; Wang, Y.S.; Lv, S.; Gao, M.; Zeng, L.Q.; Rao, Y. Simultaneous determination of 9 major components of dachengqi tang in rat plasma by UPLC-MS/MS. Chin. J. Chin. Mater. Med., 2014, 39(12), 2345-2350.
[14]
Riedlinger, J.E.; Tan, P.W.; Lu, W. Ping wei san, a Chinese medicine for gastrointestinal disorders. Ann. Pharmacother., 2001, 35(2), 228-235.
[15]
Guo, Z.F.; Wang, X.B.; Luo, J.G.; Luo, J.; Wang, J.S.; Kong, L.Y. A novel aporphine alkaloid from Magnolia officinalis. Fitoterapia, 2011, 82(4), 637-641.
[16]
Yan, R.; Wang, W.; Guo, J.; Liu, H.; Zhang, J.; Yang, B. Studies on the alkaloids of the bark of Magnolia officinalis: isolation and on-line analysis by HPLC-ESI-MS(n). Molecules, 2013, 18(7), 7739-7750.
[17]
Youn, U.J.; Chen, Q.C.; Jin, W.Y.; Lee, I.S.; Kim, H.J.; Lee, J.P.; Chang, M.J.; Min, B.S.; Bae, K.H. Cytotoxic lignans from the stem bark of Magnolia officinalis. J. Nat. Prod., 2007, 70(10), 1687-1689.
[18]
Yan, R.Y.; Liu, H.L.; Zhang, J.Y.; Yang, B. Phenolic glycosides and other constituents from the bark of Magnolia officinalis. J. Asian Nat. Prod. Res., 2014, 16(4), 400-405.
[19]
Xue, Z.Z.; Yan, R.; Yang, B. Phenylethanoid glycosides and phenolic glycosides from stem bark of Magnolia officinalis. Phytochemistry, 2016, 127, 50-62.
[20]
Yu, S.X.; Yan, R.Y.; Liang, R.X.; Wang, W.; Yang, B. Bioactive polar compounds from stem bark of Magnolia officinalis. Fitoterapia, 2011, 83(2), 356-361.
[21]
Chen, J.W.; Yang, Z.N.; Hu, T.K.; Wang, F.Y.; Sun, J.W.; Sun, M. Study on Accumulation of Seven Secondary Metabolites of Magnolia officinalis var. biloba Rehd. et Wils. with Different Tree Ages. J. Southwest Univ. Nat. Sci., 2013, 35(2), 27-34.
[22]
Zhang, S.J.; Zhong, L.Y. Advances in research of chemical constituents and pharmacological effects of Magnoliae Officinalis Cortex. J. Chin. Med. Mater, 2013, 36(5), 838-843.
[23]
Hou, X.; Yuan, X.; Zhang, B.; Wang, S.; Chen, Q. Screening active anti-breast cancer compounds from Cortex Magnolia officinalis by 2D LC-MS. J. Sep. Sci., 2013, 36(4), 706-712.
[24]
Zhang, S.J.; Zhong, L.Y. Effects of different kinds of magnolia officinalis on gastrointestinal motility. J. Chin. Med. Mater., 2014, 37(10), 1762-1765.
[25]
Cao, A.H.; Vo, L.T.; King, R.G. Honokiol protects against carbon tetrachloride induced liver damage in the rat. Phytother. Res., 2005, 19(11), 932-937.
[26]
Lee, Y.K.; Yuk, D.Y.; Kim, T.I.; Kim, Y.H.; Kim, K.T.; Kim, K.H.; Lee, B.J.; Nam, S.Y.; Hong, J.T. Protective effect of the ethanol extract of Magnolia officinalis and 4-O-methylhonokiol on scopolamine-induced memory impairment and the inhibition of acetylcholinesterase activity. J. Nat. Med., 2009, 63(3), 274-282.
[27]
Wang, X.; Wang, Y.; Geng, Y.; Li, F.; Zheng, C. Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography. J. Chromatogr. A, 2004, 1036(2), 171-175.
[28]
Xue, Z.Z.; Yan, R.Y.; Yu, S.X.; Cao, Y.Y.; Shao, A.J.; Yang, B. Determination of six active compounds in commercial samples of magnoliae officinalis cortex by HPLC-DAD. Chin. J. Exp. Tradit. Med. Form., 2009, 20(22), 45-49.
[29]
Zhang, L.L.; Bai, Y.L.; Shu, S.L.; Qian, D.W.; Ou-yang, Z.; Liu, L.; Duan, J.A. Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry. J. Sep. Sci., 2014, 37(11), 1265-1275.
[30]
Zhang, Y.; Guo, L.; Duan, L.; Dong, X.; Zhou, P.; Liu, E.H.; Li, P. Simultaneous determination of 16 phenolic constituents in Spatholobi Caulis by high performance liquid chromatography/electrospray ionization triple quadrupole mass spectrometry. J. Pharm. Biomed. Anal., 2015, 102(102), 110-118.
[31]
Harmita1; Suryadi, H.; Setiawati, A.; Damayanti, D. Development and validation of a method for simultaneous quantification of seven water-soluble vitamins in pediatrics syrup by UPLC-MS/MS. Curr. Pharm. Anal., 2018, 14(1), 23-34.

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