Noninvasive Genotyping of Dopamine Receptor D4 (DRD4) UsingNanograms of DNA From Substance-Dependent Patients

K.      Boor; Z.      Ronai; Z.      Nemoda; P.      Gaszner; M.      Sasvari-Szekely; A.      Guttman; H.      Kalasz

doi:10.2174/0929867024606821

Abstract

A noninvasive DNA sampling method has been implemented collecting buccalmucosa cells by cotton wool swabs. An amount of 0.2-2 μg DNA per patient wasobtained after the phenol-extraction procedure and 0.2-2 ng DNA template wassufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopaminereceptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separationby laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drugdependentsubjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variantsbeside the common 4-repeat DRD4 allele.

Keywords: doamine d4 recetor, genotyping, noninvasive dna samling, buccal swab

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Current Medicinal Chemistry

Title: Noninvasive Genotyping of Dopamine Receptor D4 (DRD4) UsingNanograms of DNA From Substance-Dependent Patients

Volume: 9 Issue: 8

Author(s): K. Boor, Z. Ronai, Z. Nemoda, P. Gaszner, M. Sasvari-Szekely, A. Guttman and H. Kalasz

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Keywords: doamine d4 recetor, genotyping, noninvasive dna samling, buccal swab

Abstract: A noninvasive DNA sampling method has been implemented collecting buccalmucosa cells by cotton wool swabs. An amount of 0.2-2 μg DNA per patient wasobtained after the phenol-extraction procedure and 0.2-2 ng DNA template wassufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopaminereceptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separationby laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drugdependentsubjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variantsbeside the common 4-repeat DRD4 allele.

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Boor K., Ronai Z., Nemoda Z., Gaszner P., Sasvari-Szekely M., Guttman A. and Kalasz H., Noninvasive Genotyping of Dopamine Receptor D4 (DRD4) UsingNanograms of DNA From Substance-Dependent Patients, Current Medicinal Chemistry 2002; 9 (8) . https://dx.doi.org/10.2174/0929867024606821