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Current HIV Research

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ISSN (Print): 1570-162X
ISSN (Online): 1873-4251

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Review Article

PCR Amplification Strategies Towards Full-length HIV-1 Genome Sequencing

Author(s): Chao Chun Liu and Hezhao Ji*

Volume 16, Issue 2, 2018

Page: [98 - 105] Pages: 8

DOI: 10.2174/1570162X16666180626152252

Price: $65

Abstract

The advent of next-generation sequencing has enabled greater resolution of viral diversity and improved feasibility of full viral genome sequencing allowing routine HIV-1 full genome sequencing in both research and diagnostic settings. Regardless of the sequencing platform selected, successful PCR amplification of the HIV-1 genome is essential for sequencing template preparation. As such, full HIV-1 genome amplification is a crucial step in dictating the successful and reliable sequencing downstream. Here we reviewed existing PCR protocols leading to HIV-1 full genome sequencing. In addition to the discussion on basic considerations on relevant PCR design, the advantages as well as the pitfalls of the published protocols were reviewed.

Keywords: HIV-1, Full genome, PCR, Methods, Sequencing, Next generation sequencing.

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