[1]
Wang, A.L.; Yang, M.H.; Liu, J.Q. Molecular phylogeny, recent radiation and evolution of gross morphology of the rhubarb genus Rheum (Polygonaceae) inferred from chloroplast DNA trnL-F sequences. Ann. Botan., 2005, 96, 489-498.
[2]
China Pharmacopoeia Committee. Pharmacopoeia of the People’s Republic of China; Beijing, 2010.
[3]
Wang, X.M.; Hou, X.Q.; Zhang, Y.Q.; Yang, R.; Feng, S.F. Genetic diversity of the endemic and medicinally important plant Rheum officinale as revealed by inter-simple sequence repeat (ISSR) markers. Int. J. Mol. Sci., 2012, 13, 3900-3915.
[4]
Hu, Y.P.; Wang, L.; Xie, X.L.; Yang, J.; Li, Y.; Zhang, H.G. Genetic diversity of wild populations of Rheum tanguticum endemic to China as revealed by ISSR analysis. Biochem. Syst. Ecol., 2010, 38, 264-274.
[5]
Wang, X.M.; Yang, R.; Feng, S.F.; Hou, X.Q.; Zhang, Y.Q.; Li, Y.; Ren, Y. Genetic variation in Rheum palmatum and Rheum tanguticum (polygonaceae), two medicinally and endemic species in China using ISSR markers. PLoS One, 2012, 12e51667
[6]
Barrett, S.C.H.; Kohn, J.R. Genetic and evolutionary consequences of small population size in plants, implications for conservation. Genetics and conservation of rare plants New York; , 1991.
[7]
Jin, W.; Tu, P.F. Preparative isolation and purification of trans-3,5,40-trihydroxystilbene 40-O-beta-D-glucopyranoside and catechin from Rheum tanguticum Maxim. ex Balf. using high-speed counter-current chromatography by stepwise elution and stepwise increasing the flow-rate of the mobile phase. J. Chromatogr., 2005, 1092, 241-245.
[8]
Jin, W.; Ge, R.L.; Wei, Q.J.; Bao, T.Y.; Shi, H.M.; Tu, P.F. Development of high-performance liquid chromatographic fingerprint for the quality control of Rheum tanguticum Maxim. ex Balf. J. Chromatog. A, 2006, 1132, 320-324.
[9]
Komatsu, K.; Nagayama, Y.; Tanaka, K.; Ling, Y.; Basnet, P.; Meselhy, M.R. Development of a high performance liquid chromatographic method for systematic quantitative analysis of chemical constituents in rhubarb. Chem. Pharm. Bull., 2006a, 54, 941-947.
[10]
Wang, X.; Hattori, M.; Toriizuka, K.; Terasawa, K.; Lou, Z.C.; Namba, T. Pharmacognostical studies on Chinese crude drug da-huang rhubarb V, Effect of aqueous extracts of Rheum-spp on human platelet aggregation. Shoyakugaku Zasshi, 1991, 45, 57-61.
[11]
Zhang, H.X.; Liu, M.C. Separation procedures for the pharmacologically active components of rhubarb. Chromatogr. B-Ana. Technol. Biomed. Life Sci., 2004, 81, 175-181.
[12]
Liu, W.S.; Dong, M.; Song, Z.P.; Wei, W. Genetic diversity pattern of Stipa purpurea populations in the hinterland of Qinghai-Tibet Plateau. Ann. Appl. Biol., 2009, 154, 57-65.
[13]
Zhao, Y.L.; Wang, J.B.; Zhou, G.D.; Shan, L.M.; Xiao, X.H. Investigations of free anthraquinones from rhubarb against alpha-naphthylisothiocyanate induced cholestatic liver injury in rats. Basic Clin. Pharmacol. Toxicol., 2009, 104, 463-469.
[14]
Joseph, C.; Kuhl, L.; Veronica, L. Genetic diversity of rhubarb cultivars. J. Am. Soc. Hortic. Sci., 2008, 4, 587-592.
[15]
Chen, F.J.; Wang, A.L.; Chen, K.M.; Wan, D.S.; Liu, J.Q. Genetic diversity and population structure of the endangered and medically important Rheum tanguticum (Polygonaceae) revealed by SSR markers. Biochem. Syst. Ecol., 2009, 37, 613-621.
[16]
Hilu, K.; Liang, H. The matK gene, sequence variation and application in plant systematics. Am. J. Bot., 1997, 6, 830-839.
[17]
Cao, H.; Sasaki, Y.; Fushimi, H.; Komatsu, K. Molecular analysis of medicinally-used Chinese and Japanese curcuma based on 18 s rRNA gene and trnK gene sequences. Biol. Pharm. Bull., 2001, 24, 1389-1394.
[18]
Zhu, S.; Fushimi, H.; Cai, S.Q.; Komatsu, K. Phylogenetic relationship in the genus panax, inferred from chloroplast trnK gene and nuclear 18S rRNA gene sequences. Planta Med., 2003, 69, 647-653.
[19]
Yang, D.Y.; Hirotoshi, F.; Cai, S.Q.; Katsuko, K. Molecular analysis of Rheum species used as Rhei Rhizoma based on the chloroplast matK gene sequence and its application for identification. Biol. Pharm. Bull., 2004, 3, 375-383.
[20]
Koch, M.; Haubold, B.; Mitchell-Olds, T. Molecular systematics of the Brassicaceae, evidence from coding plastidic matK and nuclear Chs sequences. Am. J. Bot., 2001, 3, 534-544.
[21]
Rofers, S.O.; Bebdich, A.J. Extraction of DNA from Plant Tissues. Plant Mol. Biol., 1998, A6, 1-10.
[22]
Thompson, J.D.; Gibson, T.J.; Plewniak, F.; Jeanmougin, F.; Higgins, D.G. The CLUSTAL_X windows interface, flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res., 1997, 25, 4876-4882.
[23]
Hall, T.A. BioEdit, a user-friendly biological sequence alignment editorand analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser., 1999, 41, 95-98.
[24]
Rozas, J.; Sanchez-DelBarrio, J.C.; Messeguer, X. DNASP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 2003, 19, 2496-2497.
[25]
Pons, O.; Petit, R.J. Measuring and testing genetic differentiation with ordered versus unordered alleles. Genetics, 1996, 144, 1237-1245.
[26]
Nei, M. Molecular population genetics and evolution Amsterdam; North-Holland Publishing Company: Oxford, 1975.
[27]
Excoffier, L.; Laval, G.; Schneider, S. Arlequin version 3.1, an integrated software package for population genetics data analysis. Evol. Bioinform. Online, 2005, 1, 47-50.
[28]
Tamura, K.; Peterson, D.; Peterson, N.; Stecher, G.; Nei, M.; Kumar, S. MEGA5, Molecular evolutionary denetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol., 2011, 10, 2731-2739.
[29]
Kimura, M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol., 1980, 16, 111-120.
[30]
Amos, W.; Harwood, J. Factors affecting levels of genetic diversity in natural populations. Philos. T. Roy. Soc. B, 1998, 1366, 177-186.
[31]
Joshi, S.P.; Gupta, V.S.; Aggarwal, R.K.; Ranjekar, P.K.; Brar, D.S. Genetic diversity and phylogenetic relationship as revealed by Inter Simple Sequence Repeat (ISSR) polymorphism in the genus Oryza. Theor. Appl. Genet., 2000, 100, 1311-1320.
[32]
Hamrick, J.L.; Godt, M.J.W. Effects of life history traits on genetic diversity in plant species. Philos. Transac. Roy. Soc. Lond. Ser. B: Biol. Sci., 1996, 351, 1291-1298.
[33]
Zheng, H.J.; Wang, H.; Yang, H.; Wu, J.H.; Shi, B.; Cai, R.; Xu, Y.B.; Wu, A.Z.; Luo, L.J. Genetic diversity and molecular evolution of Chinese waxy maize germplasm. PLoS One, 2013, 8e66606
[34]
Wang, X.M.; Hou, X.Q.; Zhang, Y.Q.; Li, Y. Distribution pattern of genuine species of rhubarb as traditional Chinese medicine. J. Med. Plants Res., 2010, 4, 1865-1876.
[35]
Komatsu, K.; Nagayama, Y.; Tanaka, K.; Ling, Y.; Cai, S.Q.; Omote, T.; Meselhy, M.R. Comparative study of chemical constituents of rhubarb from different origins. Chem. Pharm. Bull., 2006b, 54, 1491-1499.
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