Alpha-lactalbumin Effect on Myo-inositol Intestinal Absorption: In vivo and In vitro

Title:Alpha-lactalbumin Effect on Myo-inositol Intestinal Absorption: In vivo and In vitro

Volume: 15 Issue: 9

Author(s): Giovanni Monastra, Yula Sambuy, Simonetta Ferruzza, Daniela Ferrari and Giulia Ranaldi*

Affiliation:

• Council for Agricultural Research and Economics, Center for Food and Nutrition, Rome,Italy

Keywords: Myo-inositol, α-lactalbumin, pharmacokinetics, healthy volunteers, bioactive peptides, bioavailability, absorption enhancers, intestinal permeability.

Abstract: Background: Myo-inositol is a natural molecule with important therapeutic applications and an impaired oral absorption may result in a reduced clinical effect. Aim of this study was to determine if the combined oral administration of α-lactalbumin and myo-inositol in healthy subjects, could increase the plasma level of myo-inositol administered alone. In vitro studies on human differentiated intestinal Caco-2 cells were also conducted to identify the mechanisms involved in myo-inositol absorption.

Objective: The in vivo study was conducted on healthy volunteers in two phases. Subjects received a single oral myo-inositol dose. After 7 days washout, the same subjects were administered a single dose of myo-inositol and α-lactalbumin. Cmax, Tmax and AUC for myo-inositol in plasma were calculated from samples collected at different times. Transepithelial myo-inositol passage, with or without addition of digested α-lactalbumin, was measured in vitro in differentiated Caco-2 cells and compared to transepithelial electrical resistance and phenol red passage.

Results: The bioavailability of myo-inositol was modified by the concomitant administration of α- lactalbumin. Although peak concentration of myo-inositol at 180 min (Tmax) was similar for both treatments, administration of α-lactalbumin with myo-inositol in a single dose, significantly increased the plasma concentrations of myo-inositol compared to when administered alone. In vitro, myo-inositol absorption in Caco-2 cells was improved in the presence of digested α-lactalbumin, and this change was associated with an increase in tight junction permeability.

Conclusion: Better myo-inositol absorption when orally administered with α-lactalbumin can be beneficial in non-responder patients. Preliminary in vitro findings suggest that peptides deriving from α- lactalbumin digestion may modulate tight junction permeability allowing increased absorption of myoinositol.

Cite this article as:

Monastra Giovanni, Sambuy Yula, Ferruzza Simonetta, Ferrari Daniela and Ranaldi Giulia*, Alpha-lactalbumin Effect on Myo-inositol Intestinal Absorption: In vivo and In vitro, Current Drug Delivery 2018; 15 (9) . https://dx.doi.org/10.2174/1567201815666180509102641