Effect of Laser Therapy on Defensins’ Gene Expression in TR146 Epithelial Cell Line

Title:Effect of Laser Therapy on Defensins’ Gene Expression in TR146 Epithelial Cell Line

Volume: 25 Issue: 4

Author(s): Luisa Zupin*, Katia Rupel, Giulia Ottaviani, Augusto Poropat, Roberto Di Lenarda, Matteo Biasotto and Sergio Crovella

Affiliation:

• Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste,Italy

Keywords: Innate immunity, β-defensins, gene expression, laser therapy, oral mucositis, real time PCR.

Abstract: Introduction: Laser Therapy (LT) has been employed for the treatment of Oral Mucositis (OM) due to its anti-inflammatory, analgesic and bio-stimulatory effects.

Objective: This pilot study investigated the effect of LT on the expression of DEFB1, DEFB4, DEFB103 genes encoding for the human β-defensins 1, 2 and 3.

Materials and Method: TR146 epithelial cell line, used to mimic oral mucosa, was irradiated with different LT protocols. β-defensins gene expression was evaluated using TaqMan probes on Real- Time PCR platform.

Results and Conclusion: In this cellular model LT decreased mRNA defensins' expression 30 minutes after irradiation but not 24 hours later, suggesting that LT is able to influence β -defensins production immediately after treatment, while its efficacy decreases over time.

Cite this article as:

Zupin Luisa *, Rupel Katia , Ottaviani Giulia , Poropat Augusto , Di Lenarda Roberto , Biasotto Matteo and Crovella Sergio , Effect of Laser Therapy on Defensins’ Gene Expression in TR146 Epithelial Cell Line, Protein & Peptide Letters 2018; 25 (4) . https://dx.doi.org/10.2174/0929866525666180221123249

DOI https://dx.doi.org/10.2174/0929866525666180221123249	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305