Improved Scalable Synthesis and Biological Activity of “Indoxyl-gal,” a Chromogenic Histochemical Used for the Identification of Lac+ Organisms

Title:Improved Scalable Synthesis and Biological Activity of “Indoxyl-gal,” a Chromogenic Histochemical Used for the Identification of Lac+ Organisms

Volume: 15 Issue: 10

Author(s): Brent Banasik*, Tam Mai and Mansour Samadpour

Affiliation:

• Molecular Epidemiology, Inc., 15300 Bothell Way NE, Lake Forest Park, WA 98155,United States

Keywords: 3-Indoxyl-β-D-galactopyranoside, β-galactosidase, chromogenic dye, coliform bacteria, histochemistry, indicators.

Abstract: An improved and operationally simple synthesis of 3-indoxyl-β-D-galactopyranoside (indoxyl- gal) is disclosed. Indoxyl-gal is a sensitive chromogenic histochemical used for the identification of β-galactosidase enzymatic activity (lacZ gene). Synthesis of indoxyl-gal is improved to 25% overall yield in 5 linear steps from affordable starting materials with minimal chromatography. Biological testing confirmed the detection of β-galactosidase activity in all (100%, 19 strains) coliform bacteria using membrane filtration and modified MI agar.

Cite this article as:

Banasik Brent *, Mai Tam and Samadpour Mansour , Improved Scalable Synthesis and Biological Activity of “Indoxyl-gal,” a Chromogenic Histochemical Used for the Identification of Lac+ Organisms, Letters in Organic Chemistry 2018; 15 (10) . https://dx.doi.org/10.2174/1570178615666180212152030