Liquid Chromatographic Determination of Malondialdehyde in Plasma Samples After Liquid–Liquid Microextraction

Title:Liquid Chromatographic Determination of Malondialdehyde in Plasma Samples After Liquid–Liquid Microextraction

Volume: 14 Issue: 4

Author(s): Sajad Azizi, Jafar Soleymani, Maryam Khoubnasabjafari, Azam Samadi and Abolghasem Jouyban*

Affiliation:

• Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz,Iran

Keywords: Malondialdehyde, 2, 4-dinitrophenylhydrazine, vortex assisted liquid liquid microextraction, N2 assisted liquid liquid microextraction, validation, samples.

Abstract: Background: Malondialdehyde is a product of lipid peroxidation of polyunsaturated fatty acids in foods and biological samples and widely used as biomarker of oxidative stress in various diseases. We describe the validation of a new microextraction-LC-UV method for the determination of malondialdehyde (MDA) in the form of 2,4-dinitrophenylhydrazine (DNPH) derivatization in plasma samples.

Objective: A new microextraction technique called vortex and N2 assisted liquid-liquid microextraction has been developed for the determination MDA in plasma samples.

Method: The DNPH-derivatized MDA was extracted into ethyl acetate as an extraction solvent and measured by LC-UV at 310 nm after evaporation of ethyl acetate and re-dissolving in mobile phase composed of 0.2% acetic acid–acetonitrile (50:50; v/v). This method validated according to U.S. Food and Drug Administration guidelines.

Results: The limit of detection of MDA was 0.038 µmol L-1 (2.74 µg L-1) and the intra and interday relative standard deviations were in the range of 3.8-5.0% and 5.5-9.4%, respectively for different concentrations of MDA.

Conclusion: A precise and valid method to measure MDA as DNPH derivatization in plasma samples using LC was proposed.

Cite this article as:

Azizi Sajad , Soleymani Jafar , Khoubnasabjafari Maryam , Samadi Azam and Jouyban Abolghasem *, Liquid Chromatographic Determination of Malondialdehyde in Plasma Samples After Liquid–Liquid Microextraction, Current Analytical Chemistry 2018; 14 (4) . https://dx.doi.org/10.2174/1573411013666170703162443