Development of Poly(lactide-co-glicolide) Nanoparticles Incorporating Morphine Hydrochloride to Prolong its Circulation in Blood

Title:Development of Poly(lactide-co-glicolide) Nanoparticles Incorporating Morphine Hydrochloride to Prolong its Circulation in Blood

Volume: 23 Issue: 13

Author(s): Victoria Gomez-Murcia, Mercedes Garcia Montalban, Juan C. Gomez-Fernandez*Pilar Almela

Affiliation:

• Departamento de Bioquímica y Biología Molecular A, Universidad de Murcia, Murcia, IMIB-Arrixaca, Regional Campus of International Excellence Campus Mare Nostrum, Facultad de Veterinaria Campus de Espinardo. 30100. Murcia,Spain

Keywords: Morphine, nanoparticles, PLGA, pharmacokinetics, lyophilized, poly(lactide-co-glicolide) (PLGA).

Abstract: Background: Formulations incorporating nanoparticles (NPs) are widely used to prolong drug release. In this regard, poly(lactide-co-glicolide) (PLGA) is often used in their preparation due to its high degree of biocompatibility and biodegradability. In the present study, morphine HCl is incorporated in PLGA-NPs and different preparation alternatives are evaluated for their effects on the properties, stability and capacity of encapsulation.

Methods: NPs were prepared by a double emulsion solvent diffusion-ammonium loading (DESD-AL) or double emulsion solvent diffusion-traditional (DESD-T) technique. NP morphology, size, zeta potential and encapsulation efficiency were investigated. In vitro studies were performed in phosphate buffer pH 7.4 at 37 ºC and deionized water at 4ºC. Adult male Swiss mice were used to study the pharmacokinetic behavior in vivo.

Results: Our results show that DESD-AL provides a higher level of morphine entrapment and that increasing the sonication time reduces the size but does not appreciably reduce the entrapment percentage. It was also observed that NP stability was greater when Pluronic F68 was used rather than PVA, and that in vitro assays provided better results with low concentrations of both stabilizers. Lyophilized NPs, after rehydration showed properties that were only slightly different from those of the untreated ones, with no sign of precipitation or aggregation. Finally, the obtained NPs enhanced morphine bioavailability.

Conclusions: In conclusion, a useful method for encapsulating morphine in order to obtain an extended delivery period is described and its effects are compared with those of the free drug.

Cite this article as:

Gomez-Murcia Victoria, Montalban Garcia Mercedes, Gomez-Fernandez C. Juan*, Almela Pilar, Development of Poly(lactide-co-glicolide) Nanoparticles Incorporating Morphine Hydrochloride to Prolong its Circulation in Blood, Current Pharmaceutical Design 2017; 23 (13) . https://dx.doi.org/10.2174/1381612822666161201152604

DOI https://dx.doi.org/10.2174/1381612822666161201152604	Print ISSN 1381-6128
Publisher Name Bentham Science Publisher	Online ISSN 1873-4286