Abstract
Background: A rapid stability-indicating UHPLC-DAD method was developed and validated for the determination of rutin in a bulk drug and diet supplements in the presence of related substances (impurities, degradation products, other active substances and excipients).
Experiment: A Kinetex C-18 (100 mm × 2.10 mm, 1.7 µm) column with semi-porous filling and the following separation conditions were used: mobile phase, acetonitrile–0.1% formic acid (20:80 V/V); flow rate, 0.4 mL/min; detection wavelength, 353 nm; column temperature, 298 K. The UHPLC-DAD method was linear in the range 0.20–1.40 µg with the correlation coefficients 0.9993 (rutin), 0.9995 (isoquercetin) and 0.9991 (quercetin), where accuracy and precision (intra- and inter-day) were acceptable. The limits of detection were 0.0436 µg (rutin), 0.0393 µg (isoquercetin) and 0.0442 µg (quercetin) whereas the limits of quantification were 0.1322 µg (rutin), 0.1192 µg (isoquercetin) and 0.1339 µg (quercetin). Conclusion: The determination of rutin in the presence of its degradation products by using the UHPLCDAD method forming in aqueous solutions during acidic-basic hydrolysis and oxidation as well as in solid state during photolysis and thermolysis were studied. The degradation of rutin was a first-order reaction depending on the substrate concentration.Keywords: Rutin, isoquercetin, quercetin, UHPLC-DAD, diet supplement, stability.
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