Cytotoxic and Chemopreventive Effects of Gemin D Against Different Mutagens Using In Vitro and In Vivo Assays

Title:Cytotoxic and Chemopreventive Effects of Gemin D Against Different Mutagens Using In Vitro and In Vivo Assays

Volume: 17 Issue: 5

Author(s): Cristiene Costa Carneiro*, Aroldo Vieira de Moraes-Filho, Amanda Silva Fernandes, Suzana da Costa Santos, Daniela de Melo e Silva and Lee Chen Chen

Affiliation:

• Department of Genetics, Institute of Biological Sciences, Federal University of Goias, C.P. 131, Campus Samambaia, P.O.Box 74001-970, Goiania,Brazil

Keywords: Ellagitannin, gemin D, cytotoxic and protective effects against different mutagens, ames test, micronucleus test, comet assay.

Abstract: Background: Gemin D (GD) is an ellagitannin found in several plant species rich in phenolic compounds. Its many beneficial properties include antioxidant and antitumoral.

Objective: The present study assessed the genotoxicity, cytotoxicity, antigenotoxicity, and anticytotoxicity of GD by in vitro and in vivo assays.

Method: The Ames mutagenicity assay in Salmonella typhimurium, Micronucleus and Comet tests in mice were used to evaluate the biological activities mentioned above. To assess the GD’s protective effects against DNA damage induced by different mutagens we performed co-, pre- and/or post-treatment in these assays.

Results: There was no genotoxic effect of GD via Ames and Micronucleus tests, but in the Comet assay the highest dose induced DNA damage. This same highest dose presented a significant cytotoxicity in mice. In the antigenotoxicity, GD protected DNA against the action of 4-nitroquinoline-1-oxide and sodium azide by Ames test, and also against the harmful action of cyclophosphamide in pre- and co-treatment by Micronucleus and Comet tests, but it did not protect DNA in post-treatment. Regarding to anticytotoxicity, GD provoked an anticytotoxic effect only during pre-treatment.

Conclusion: Therefore, GD showed relevant antigenotoxic, anticytotoxic and cytotoxic effects, which indicate that it may be a probable candidate for chemoprevention or for the development of new cancer therapies.

Cite this article as:

Carneiro Costa Cristiene*, Moraes-Filho Vieira de Aroldo, Fernandes Silva Amanda, Santos da Costa Suzana, Silva de Melo e Daniela and Chen Chen Lee, Cytotoxic and Chemopreventive Effects of Gemin D Against Different Mutagens Using In Vitro and In Vivo Assays, Anti-Cancer Agents in Medicinal Chemistry 2017; 17 (5) . https://dx.doi.org/10.2174/1871520616666160906092502

DOI https://dx.doi.org/10.2174/1871520616666160906092502	Print ISSN 1871-5206
Publisher Name Bentham Science Publisher	Online ISSN 1875-5992