长寿保障5与琥珀酸脱氢酶B亚单位协同抑制p53和p21活性

Title:LASS5 Interacts with SDHB and Synergistically Represses p53 and p21 Activity

Volume: 16 Issue: 6

Author(s): Z. Jiang, F. Li, Y. Wan, Z. Han, W. Yuan, L. Cao, Y. Deng, X. Peng, F. Chen, X. Fan, X. Liu, G. Dai, Y. Wang, Q. Zeng and Y. Shi, Z. Zhou, Y. Chen, W. Xu, S. Luo, S. Chen, X. Ye, X. Mo, X. Wu, Y. Li

Affiliation:

关键词: 长寿保障5，琥珀酸脱氢酶B亚单位，神经胶质瘤，p53。

摘要: Longevity Assurance 5 (LASS5), a member of the LASS/Ceramide Synthases family, synthesizes C16-ceramide and is implicated in tumor biology. However, its precise role is not yet well understood. A yeast two-hybrid screen was performed using a human cDNA library to identify potential LASS5- interaction partners. One identified clone encodes succinate dehydrogenase subunit B (SDHB). Mammalian two-hybrid assays showed that LASS5 interacts with SDHB, and the result was also confirmed by GST pull-down and coimmunoprecipitation assays. The C-terminal fragment of SDHB was required for the interaction. LASS5 and SDHB were co-localized in COS-7 cells. LASS5 and SDHB expressions were found to be up-regulated in neuroglioma tissue. Transfection assays showed that LASS5 or SDHB expression repressed p53 or p21 reporter activity, respectively. Simultaneous LASS5 and SDHB expression resulted in stronger repression of p53 and p21 reporter activity, suggesting that LASS5 and SDHB interaction may synergistically affect transcriptional regulation of p53 and p21. Our data provide new molecular insights into potential roles of LASS5 and SDHB in tumor biology.

Cite this article as:

Z. Jiang, F. Li, Y. Wan, Z. Han, W. Yuan, L. Cao, Y. Deng, X. Peng, F. Chen, X. Fan, X. Liu, G. Dai, Y. Wang, Q. Zeng and Y. Shi, Z. Zhou, Y. Chen, W. Xu, S. Luo, S. Chen, X. Ye, X. Mo, X. Wu, Y. Li , LASS5 Interacts with SDHB and Synergistically Represses p53 and p21 Activity, Current Molecular Medicine 2016; 16 (6) . https://dx.doi.org/10.2174/1566524016666160607090012