N-Terminal Cys-Rich Region of a Rice Type 1 Metallothionein Independently Forms Metal-Thiolate Cluster

Title:N-Terminal Cys-Rich Region of a Rice Type 1 Metallothionein Independently Forms Metal-Thiolate Cluster

Volume: 23 Issue: 7

Author(s): Soheila Ahmadi and Azar Shahpiri

Affiliation:

Keywords: Metallothionein, Metal-thiolate cluster, N-terminal Cys-rich region, OsMTI-1b, Rice.

Abstract: The members of plant metallothionein (MT) subfamily p1 are characterized with the presence of six Cys at each end of N- and C-terminal of their amino acid sequences which are arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC sequence, respectively. In this study we evaluated the independence of N-terminal Cys-rich region of a type 1 MT isoform from rice (OsMTI- 1b) in forming metal-thiolate cluster. To this end the N-terminal of OsMTI-1b (N-OsMTI-1b) was heterologously expressed in Escherichia coli as fusion protein with glutathione-S-transferase (GST). The E.coli cells expressing GST-N-OsMTI-1b were able to remove Cd²⁺ and Ni²⁺ from culture medium. The recombinant GST-N-OsMTI-1b was purified using affinity chromatography. The UV absorption spectra recorded after the reconstitution of the apo-protein with Cd²⁺ and Ni²⁺ confirmed that GST-N-OsMTI-1b was able to form complexes with Cd²⁺ and Ni²⁺. These results demonstrate the formation of independent metal-thiolate cluster at Nterminal Cys-rich region of GST-N-OsMTI-1b without participation of C-terminal Cys-rich region.

Cite this article as:

Ahmadi Soheila and Shahpiri Azar, N-Terminal Cys-Rich Region of a Rice Type 1 Metallothionein Independently Forms Metal-Thiolate Cluster, Protein & Peptide Letters 2016; 23 (7) . https://dx.doi.org/10.2174/0929866523666160511150730

DOI https://dx.doi.org/10.2174/0929866523666160511150730	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305