Rapid Estimation of Beta-Lactam Antibiotics in Biological Fluids

Title:Rapid Estimation of Beta-Lactam Antibiotics in Biological Fluids

Volume: 4 Issue: 2

Author(s): Bernard Joris, Jacques Degelaen, Frank Klein and Jean-Marie Frere

Affiliation:

Keywords: Beta-lactam antibiotics, penicillin-sensitive enzyme

Abstract: The Actinomadura R39 DD-peptidase is a very penicillin-sensitive enzyme. After a short time of contact with a liquid sample containing a beta-lactam antibiotic, the determination of the residual enzyme activity allows estimation of the concentration of the antibiotic in the sample. An alternative method utilizes the C-terminal domain of the Bacillus licheniformis penicillin sensor BlaR that is devoid of enzymatic activity. In this case, the protein is labelled with gold particles and the unreacted portion is captured by beta-lactam molecules covalently attached to a solid support. Both methods have been quite successful for the rapid estimation of beta-lactam antibiotics in milk.

Cite this article as:

Joris Bernard, Degelaen Jacques, Klein Frank and Frere Jean-Marie, Rapid Estimation of Beta-Lactam Antibiotics in Biological Fluids, Current Biotechnology 2015; 4 (2) . https://dx.doi.org/10.2174/2211550104666150624171854