Serum of Blood Separation by Means of Dynamic Electrochromatography in a Centrifugal and Electromagnetic Gradient

Title:Serum of Blood Separation by Means of Dynamic Electrochromatography in a Centrifugal and Electromagnetic Gradient

Volume: 8 Issue: 3

Author(s): Andrzej Jan Dyszkiewicz

Affiliation:

Keywords: Centrifugal gradient, electric centripetal force, electromagnetic gradient, electrochromatography, multigradient electrochromatography, quantum-preparative techniques, quick analytic systems, radial separation phase, relaxation electrical dipole, relaxation spin imbalance, steering buffer parameters, transversal separation phase.

Abstract: The paper presents the dynamic association of several gradients of separation with electrophoresis and the laboratory implementation of a prototype device for dynamic electrochromatography in a centrifugal and electromagnetic gradient (DECGOE), based on patent PL171643. The object of the study was to compare the separation capacity and repeatability of the location of edge parameters (xx₍₁₎, yy₍₁₎, d) of the contours of a proteinogram fraction for conventional electrophoresis (EF), radial SR-DECGOE separation and three variants of radial-transverse SR-SPM, SR-SPE, SRSPRRM distribution as well as the reproducibility of the following prototype parameters: PV₍₁₎, PV₍₂₎, PV₍₃₎[V] potentials, PV_(3)[F] frequency, ω₍₁₎ and ω₍₂₎ rotational speed, MC mixer chamber buffer [pH] and volume[ml], and B [T] magnetic induction. Human blood serum was used in the study, 50 samples for each separation type. The highest rate of fraction formation was registered for the SR-SPRRM (8,07/s) variant. The percentage values of standard deviations for the edge parameters of proteinograms were the lowest for conventional electrophoresis (EF), slightly higher for radial distribution SR, while among the radial-transverse separations, the highest accuracy was obtained for the SR-SPRRM variant.

Cite this article as:

Jan Dyszkiewicz Andrzej, Serum of Blood Separation by Means of Dynamic Electrochromatography in a Centrifugal and Electromagnetic Gradient, Recent Patents on Biotechnology 2014; 8 (3) . https://dx.doi.org/10.2174/187220830803150605163521