Towards Point-of-Use Dielectrophoretic Methods: A New Portable Multiphase Generator for Bacteria Concentration

Title:Towards Point-of-Use Dielectrophoretic Methods: A New Portable Multiphase Generator for Bacteria Concentration

Volume: 6 Issue: 2

Author(s): B. del Moral Zamora, J.M. Alvarez Azpeitia, J. Colomer-Farrarons, P.Ll. Miribel-Catala, A. Homs-Corbera, A. Juarez and J. Samitier

Affiliation:

Keywords: Cell Concentrator, Class E amplifier, Dielectrophoresis, electronics, lab-on-a-chip (LOC), low cost, portable device.

Abstract: This manuscript presents a portable and low cost electronic system for specific point-of-use dielectrophoresis applications. The system is composed of two main modules: a) a multiphase generator based on a Class E amplifier, which provides 4 sinusoidal signals (0°, 90°, 180°, 270°) at 1 MHz with variable output voltage up to 10 Vpp (Vm) and an output driving current of 1 A; and b) a dielectrophoresis-based microfluidic chip containing two interdigitated electrodes. The system has been validated by concentrating Escherichia coli (E. coli) at 1 MHz while applying a continuous flow of 5 µL/min. The device functionalities were verified under different conditions, achieving an 83% trapping efficiency when counter-phased signals are used.

Cite this article as:

Zamora del Moral B., Azpeitia Alvarez J.M., Colomer-Farrarons J., Miribel-Catala P.Ll., Homs-Corbera A., Juarez A. and Samitier J., Towards Point-of-Use Dielectrophoretic Methods: A New Portable Multiphase Generator for Bacteria Concentration, Micro and Nanosystems 2014; 6 (2) . https://dx.doi.org/10.2174/187640290602141127113559