A New Quaternary Ammonium Cysteine Analogue as a Trapping Reagent for Reactive Metabolites Screening

Title:A New Quaternary Ammonium Cysteine Analogue as a Trapping Reagent for Reactive Metabolites Screening

Volume: 8 Issue: 1

Author(s): Natalia Penner, Xiaojing Liu and Chandra Prakash

Affiliation:

Keywords: Bioactivation, cysteine-cholamine, glutathione, LC-MS/MS, metabolism, reactive metabolite screening, trapping reagent.

Abstract: The novel cysteine-cholamine (Cys-chol) trapping reagent was synthesized by coupling N-(tertbutoxycarbonyl)- S-trityl-L-cysteine with cholamine in the presence of HBTU (O-(benzotriazol-1-yl)- N,N,N',N'-tetramethyluronium hexafluorophosphate), and then deprotecting by trifluoroacetic acid. Cys-chol reagent enhanced the sensitivity of reactive metabolite screening 4 to 20 times without introducing additional sample preparation or derivatization steps. Retention of Cys-chol conjugates on reversed-phase column is higher than for respective GSH conjugates which helps in reduction of background interference. The use of Cys-chol trapping reagent can potentially improve sensitivity and specificity of routine reactive metabolite screening assay in drug discovery.

Cite this article as:

Penner Natalia, Liu Xiaojing and Prakash Chandra, A New Quaternary Ammonium Cysteine Analogue as a Trapping Reagent for Reactive Metabolites Screening, Drug Metabolism Letters 2014; 8 (1) . https://dx.doi.org/10.2174/1872312808666140606100739