TiO₂~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets

Title:TiO₂~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets

Volume: 4 Issue: 2

Author(s): Asya S. Levina, Marina N. Repkova, Nadezhda V. Shikina, Zinfer R. Ismagilov, Natalia V. Shatskaya and Valentina F. Zarytova

Affiliation:

Keywords: Delivery, deoxyribozymes, immobilization, nanocomposites, TiO₂ nanoparticles.

Abstract: Synthetic DNA molecules of 10-23 deoxyribozymes (Dz) are known to be efficient and site-specific agents for the RNA cleavage in a catalytic manner. To provide the penetration of Dz into cells, we have proposed the new delivery system for deoxyribozymes in the form of TiO₂•PL-Dz nanocomposite. These nanocomposites consist of catalytically active Dz molecules noncovalently immobilized through the polylysine linker to TiO₂ nanoparticles, which provide the penetration of Dz into cells. Deoxyribozymes in the prepared TiO₂•PL-Dz nanocomposites were shown to retain their ability to cleave RNA targets albeit at a slower rate but with the same site-specificity and similar efficiency as free Dz. Unlike free Dz, the proposed TiO₂•PL-Dz nanocomposites penetrate into cells without auxiliary actions.

Cite this article as:

Levina S. Asya, Repkova N. Marina, Shikina V. Nadezhda, Ismagilov R. Zinfer, Shatskaya V. Natalia and Zarytova F. Valentina, TiO₂~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets, Drug Delivery Letters 2014; 4 (2) . https://dx.doi.org/10.2174/2210303104666140222002510