Proteomic Study on the Protective Effect of Tanshinone IIA Against Oxidative Injury to Human Umbilical Vein Endothelial Cells by Hydrogen Peroxide

Title:Proteomic Study on the Protective Effect of Tanshinone IIA Against Oxidative Injury to Human Umbilical Vein Endothelial Cells by Hydrogen Peroxide

Volume: 10 Issue: 4

Author(s): Yao-hong Teng, De-zhao Lu, Chun-lei Fan, Ya-ting Dong and Ji Zhu

Affiliation:

Keywords: HUVEC, H₂O₂, atherosclerosis, proteome, Tanshinone IIA.

Abstract: Atherosclerosis is considered a chronic inflammatory disease and injury to vascular endothelial cells is an important event at the onset of its pathogenesis. Oxidative stress is regarded as a pivotal pathogenic factor in the development of endothelial cell injury and apoptosis. Tanshinone IIA is considered the main active lipophilic component of the traditional Chinese medicine Danshen. It has been demonstrated that Tanshinone IIA protects endothelial cells from oxidative stress-triggered injury and apoptosis, but many of its anti-oxidant and anti-apoptotic mechanisms still remain to be elucidated.

In this paper, our results show that HUVECs incubated with 1.1mM H₂O₂ for 12h had significantly decreased the viability of endothelial cells, which was accompanied with obviously cell apoptosis. However, treatment with Tanshinone IIA (8 mM, 24h) resulted in a significant resistance to H₂O₂-induced apoptosis. We used 2D-DIGE (Difference in Gel Electrophoresis) analysis followed by MALDI-TOF-TOF-MS to identify the proteins differentially expressed after human umbilical vein endothelial cells (HUVECs) were protected with Tanshinone IIA against oxidative damage by hydrogen peroxide. There were various differently expressed proteins, we picked out 32 proteins to analysis, and discovered that Tanshinone IIA could protect endothelial cells by its anti-inflammatory or antioxidant effects, regulating proliferation and differentiation, maintaining the cytoskeleton, and maintaining intracellular calcium homeostasis.

Cite this article as:

Teng Yao-hong, Lu De-zhao, Fan Chun-lei, Dong Ya-ting and Zhu Ji, Proteomic Study on the Protective Effect of Tanshinone IIA Against Oxidative Injury to Human Umbilical Vein Endothelial Cells by Hydrogen Peroxide, Current Proteomics 2013; 10 (4) . https://dx.doi.org/10.2174/1570164610999131125102832