N-Glycosylation of Plant-produced Recombinant Proteins

Title:N-Glycosylation of Plant-produced Recombinant Proteins

Volume: 19 Issue: 31

Author(s): Dirk Bosch, Alexandra Castilho, Andreas Loos, Arjen Schots and Herta Steinkellner

Affiliation:

Keywords: Plants, recombinant proteins, glycosylation, glyco-engineering.

Abstract: Plants are gaining increasingly acceptance as a production platform for recombinant proteins. One reason for this is their ability to carry out posttranslational protein modifications in a similar if not identical way as mammalian cells. The capability of plants to carry out human-like complex glycosylation is well known. Moreover, the targeted manipulation of the plant N-glycosylation pathway allows the production of proteins carrying largely homogeneous, human-type oligosaccharides. These outstanding results have placed plants in a favourable position compared to other eukaryotic expression systems. This review provides a comprehensive summary of the N-glycosylation of plant-produced recombinant proteins, the possible impact of plant-specific N-glycans on the human immune system, and recent advances in engineering the plant N-glycosylation pathway towards the synthesis of (complex) human-type glycan structures, highlighting challenges and achievements in the application of these powerful technologies.

Cite this article as:

Bosch Dirk, Castilho Alexandra, Loos Andreas, Schots Arjen and Steinkellner Herta, N-Glycosylation of Plant-produced Recombinant Proteins, Current Pharmaceutical Design 2013; 19 (31) . https://dx.doi.org/10.2174/1381612811319310006