Synthesis and Characterization of Silver Nanoparticles from Streptomyces olivaceus sp-1392 and its Anticancerous Activity Against Non-Small Cell Lung Carcinoma Cell Line (NCI-H460)

R.      Subbaiya; Masilamani      Selvam

doi:10.2174/15734137113099990073

Abstract

Anticancerous activity of silver nanoparticles, synthesized by Streptomyces olivaceus was evaluated against Non-small cell lung carcinoma cell line (NCI-H460). The optimum concentration of 1mM silver nitrate required for biological synthesis was identified by inoculating the pellet and supernatant obtained from Streptomyces olivaceus-1392 in various concentrations of Silver nitrate solution. The nanoparticles were synthesized intracellularly by inoculating the culture in 1mM silver nitrate under standard conditions and the production was determined by UV Spectrophotometer analysis. Fourier Transform Infrared Spectroscopy confirmed the presence of proteins as the stabilizing agent surrounding the Silver nanoparticles. The anticancerous activity against Non-small cell lung carcinoma cell line (NCI-H460) by MTT assay reveals that 12.52μg(IC₅₀) inhibits the NCI-H460 growth. The effect of nanoparticles on cell morphology was studied by Hoechst staining to confirm the cell death. The DNA damage in the treated cells was evaluated using single-cell gel electrophoresis (comet) assay. DNA was observed in cells treated with nanoparticles after 24h incubation whereas the percentage of damaged cells increased on treatment with this compound for 48h, which was shown by the appearance of prominent comets with tail. The study demonstrated the possible use of biologically synthesized silver nanoparticles as an effective drug against cancer. ROS generation shows the nanoparticle treatment which was measured spectrofluorimetrically through DCFH-DA staining.

Keywords: Streptomyces olivaceus, comet assay, anticancerous, MTT, ROS (Reactive Oxygen Species).

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