Analytical and Biological Evaluation of High Throughput Screen Actives Using Evaporative Light Scattering, Chemiluminescent Nitrogen Detection, and Accurate Mass LC-MS-MS

Title: Analytical and Biological Evaluation of High Throughput Screen Actives Using Evaporative Light Scattering, Chemiluminescent Nitrogen Detection, and Accurate Mass LC-MS-MS

Volume: 8 Issue: 6

Author(s): D.A. Peake, D.C. Duckworth, T.J. Perun, W.L. Scott, P. Kulanthaivel and M.A. Strege

Affiliation:

Keywords: Bioassay-guided fractionation, biofractionation evaporative light scattering detection (ELSD), evaporative light scattering detection (ELSD), chemiluminescent nitrogen detection (CLND), electrospray ionization mass spectrometry, LC-MS, accurate mass measurement, LC-MS-MS analysis, high throughput screen

Abstract: In this investigation the utility of evaporative light scattering detection (ELSD) combined with HPLC-MS was demonstrated as a key component of a bioassay-guided fractionation, or “biofractionation” technique, for the evaluation of high throughput screen actives. ELSD provided on-line analyte mass information that was critical for the classification of the samples. Chemiluminescent nitrogen detection (CLND) was also evaluated for sample concentration estimation for nitrogen-containing compounds, and accurate mass LC-MS-MS analysis was employed for rapid structural confirmation and elucidation of components previously identified as active via biofractionation.

Cite this article as:

D.A. Peake , D.C. Duckworth , T.J. Perun , W.L. Scott , P. Kulanthaivel and M.A. Strege , Analytical and Biological Evaluation of High Throughput Screen Actives Using Evaporative Light Scattering, Chemiluminescent Nitrogen Detection, and Accurate Mass LC-MS-MS, Combinatorial Chemistry & High Throughput Screening 2005; 8 (6) . https://dx.doi.org/10.2174/1386207054867346

DOI https://dx.doi.org/10.2174/1386207054867346	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402