Effects of Amino Acid Deletion and Substitution on the Chemical Properties, Biological Activities of the Frog Peptide Palustrin-OG1

Title:Effects of Amino Acid Deletion and Substitution on the Chemical Properties, Biological Activities of the Frog Peptide Palustrin-OG1

Volume: 20 Issue: 7

Author(s): Yong-Gang Xie, Yi-Fan Liu, Chao Luan, Fei-Fei Han, Ren Lai, Denis Groleau, Jie Feng and Yi-Zhen Wang

Affiliation:

Keywords: Palustrin-OG1, host defense peptide, antimicrobial activity, killing kinetics, cytotoxicity

Abstract: Palustrin-OG1 (OG1) is a host defense peptide isolated from the frog Odorrana grahami. In this study, we analyzed the chemical properties, antimicrobial activities and cytotoxicities of OG1 and its derivatives to identify the most promising peptide as an antimicrobial agent. By increasing the net positive charge, amphipathicity and decreasing the mean hydrophobicity of OG1, the derivative named as OG2 exerted higher antimicrobial activity against bacteria but lower cytotoxicity against both porcine erythrocytes and peripheral blood mononuclear cells than did OG1 (P<0.01). After substitution of Cys residues of OG2 by Ala or Trp residues, two derivatives named as OG2A and OG2W were less effective against bacteria and induced greater hemolysis than did OG2, indicating the importance of Cys residues. The substitution of the C-terminal Thr of OG2 resulted OG2N, which decreased the cytotoxicity and improved killing kinetics against gram-positive bacteria by the rapid damage of cell wall and membrane.

Cite this article as:

Xie Yong-Gang, Liu Yi-Fan, Luan Chao, Han Fei-Fei, Lai Ren, Groleau Denis, Feng Jie and Wang Yi-Zhen, Effects of Amino Acid Deletion and Substitution on the Chemical Properties, Biological Activities of the Frog Peptide Palustrin-OG1, Protein & Peptide Letters 2013; 20 (7) . https://dx.doi.org/10.2174/0929866511320070012

DOI https://dx.doi.org/10.2174/0929866511320070012	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305