Abstract
Resolution of ternary and quaternary mixtures of paracetamol (PAR), desxtromethorphan (DEX), phenylephrine (PHE), and chlorpheniramine (CHL) was successfully achieved with minimum sample pre-treatment and without analyte separation using a rapid method based on partial least squares (PLS) and UV spectral data. Multivariate PLS calibration was performed using 35 ternary and quaternary mixtures and the model was employed for prediction of analyte in 15 external test samples. Simultaneous determination of the analytes was possible by analyzing absorbances between 210 and 300 nm. Q2 values which are a measure of the predictivity of the PLS models were between 0.9696 and 0.9880 in ternary mixtures and between 0.9230 and 0.9757 in quaternary mixtures. The PLS models were successfully applied to determine the analytes in real pharmaceutical preparations. The mean recoveries for the analytes in the real samples were between 95.25 and 102.4% in ternary and between 93.52 and 103.55% in quaternary mixtures.
Keywords: Chlorpheniramine, dextromethorphan, paracetamol, phenylephrine, partial least squares, pharmaceutical
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