Coherent Anti-Stokes Raman Scattering Microscopy for High Speed Non- Staining Biomolecular Imaging

Title:Coherent Anti-Stokes Raman Scattering Microscopy for High Speed Non- Staining Biomolecular Imaging

Volume: 14 Issue: 2

Author(s): Mamoru Hashimoto, Takeo Minamikawa and Tsutomu Araki

Affiliation:

Keywords: Nonlinear microscopy, optical microscopy, raman microscopy, real-time imaging, ultrafast laser

Abstract: Vibrational microscopy (Raman microscopy and infrared microscopy), which observes molecular vibrations, gives us the information of molecular species without staining because the observed signals are originated from intrinsic molecules of a cell. However, infrared radiation is absorbed with water, and the long wavelength (3-10 μm) limits the spatial resolution to several micrometers. Spontaneous emission of Raman scattering is quite feeble, and the Raman scattering often overlaps with one-photon fluorescence from a specimen. Coherent anti-Stokes Raman scattering (CARS) microscopy, which is one of the nonlinear Raman microscopy, is a method to overcome those problems. In this review, present system of CARS microscopy, the methods of background rejection, and applications are introduced.

Cite this article as:

Hashimoto Mamoru, Minamikawa Takeo and Araki Tsutomu, Coherent Anti-Stokes Raman Scattering Microscopy for High Speed Non- Staining Biomolecular Imaging, Current Pharmaceutical Biotechnology 2013; 14 (2) . https://dx.doi.org/10.2174/1389201011314020004