Prokaryotic Expression, Purification, and Production of Glutathione S-Transferase-tagged Neural Stem Cell Specific Peptides from Phage Display Screening

Title:Prokaryotic Expression, Purification, and Production of Glutathione S-Transferase-tagged Neural Stem Cell Specific Peptides from Phage Display Screening

Volume: 20 Issue: 2

Author(s): Lei Jin, Wenxiu Zhao and Lan Ma

Affiliation:

Keywords: Phage display, prokaryotic expression, glutathione-S-transferase -tagged, neural stem cell, peptide

Abstract: Combining stem cell with phage display to discover novel biomarkers is a new field in stem cell studies. Novel peptides obtained through phage display panning have been functionally identified and involved into initial applications as cell culture support or cell label. In the present study, we designed a glutathione S-transferase (GST)-tagged peptide complex to construct an easy and efficient prokaryotic expression and purification platform for peptides obtained from phage display panning. The purified GST-peptide protein could specifically bind to neural stem (NS) cells and could be flexibly used for GST pull down assay or NS cell labeling in future studies. The results of the present study would be useful for cell labeling and future investigations of special receptors on stem cell surface.

Cite this article as:

Jin Lei, Zhao Wenxiu and Ma Lan, Prokaryotic Expression, Purification, and Production of Glutathione S-Transferase-tagged Neural Stem Cell Specific Peptides from Phage Display Screening, Protein & Peptide Letters 2013; 20 (2) . https://dx.doi.org/10.2174/0929866511320020007

DOI https://dx.doi.org/10.2174/0929866511320020007	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305