Electrochemical Oxidation of Flavonoids at a Nano- Metal Oxide Modified Carbon Paste Electrodes and Determination Their Levels in Human Urine

Title:Electrochemical Oxidation of Flavonoids at a Nano- Metal Oxide Modified Carbon Paste Electrodes and Determination Their Levels in Human Urine

Volume: 9 Issue: 1

Author(s): Lai-Hao Wang, Hsiu-Hua Liu

Affiliation:

Keywords: Nano- metal oxide, Modified carbon paste electrodes, Flow-through voltammetric detector, Flavonoids, Human urine

Abstract: We constructed nano-metal oxide (tin oxide, cerium oxide, cobalt oxide, iron oxide, nickel oxide, gold oxide, and silver oxide) -modified carbon paste electrodes (CPEs) for detecting flavonols (kaempferol, myricetin, and quercetin) and flavones (luteolin and apigenin) using high-performance liquid chromatography with an electrochemical detector (HPLC-ECD). Flavonoids are more efficiently catalytically oxidized on a gold oxid e-modified CPE than on a bare CPE. For direct current mode, with the current at a constant potential, and measurements with suitable experimental parameters, a linear concentration from 40 to 1600 ng mL-1 was found. The limits of quantification for flavonoids were below 0.5 ng. The developed method detected flavonoids in urine samples. Findings using HPLC-ECD and HPLC with an ultraviolet detector were comparable.

Cite this article as:

Lai-Hao Wang, Hsiu-Hua Liu , Electrochemical Oxidation of Flavonoids at a Nano- Metal Oxide Modified Carbon Paste Electrodes and Determination Their Levels in Human Urine, Current Analytical Chemistry 2013; 9 (1) . https://dx.doi.org/10.2174/1573411011309010143