Fluorescence Activated Cell Sorting: A Window on the Stem Cell

K.W. Johnson; M. Dooner; P.J. Quesenberry

doi:10.2174/138920107780906487

Abstract

Fluorescence activated cell sorting (FACS) in the field of stem cell biology has become an indispensable tool for defining and separating rare cell populations with a high degree of purity. Steady progress has been made in this regard, but the intrinsic lability of the stem cell phenotype presents a different challenge and there are many technical caveats. FACS remains, however, the technology of choice for reporting and characterizing rare cell populations such as stem cells.

Keywords: FACS, flow cytometry, cell sorting, stem cell, neural stem cell, progenitor cell, hematopoesis, neuropoesis

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Current Pharmaceutical Biotechnology

Title: Fluorescence Activated Cell Sorting: A Window on the Stem Cell

Volume: 8 Issue: 3

Author(s): K.W. Johnson, M. Dooner and P.J. Quesenberry

Affiliation:

Keywords: FACS, flow cytometry, cell sorting, stem cell, neural stem cell, progenitor cell, hematopoesis, neuropoesis

Abstract: Fluorescence activated cell sorting (FACS) in the field of stem cell biology has become an indispensable tool for defining and separating rare cell populations with a high degree of purity. Steady progress has been made in this regard, but the intrinsic lability of the stem cell phenotype presents a different challenge and there are many technical caveats. FACS remains, however, the technology of choice for reporting and characterizing rare cell populations such as stem cells.

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K.W. Johnson , M. Dooner and P.J. Quesenberry , Fluorescence Activated Cell Sorting: A Window on the Stem Cell, Current Pharmaceutical Biotechnology 2007; 8 (3) . https://dx.doi.org/10.2174/138920107780906487