Predicting Protein Crystallizability and Nucleation

Title:Predicting Protein Crystallizability and Nucleation

Volume: 19 Issue: 7

Author(s): Nuria Sanchez-Puig, Claude Sauter, Bernard Lorber, Richard Giege and Abel Moreno

Affiliation:

Keywords: Proteins, crystallization feasibility, crystal growth, X-ray diffraction, nucleation, surface energy, 3D structure , physico-chemical properties, crystallization propensity, amino acids

Abstract: The outcome of protein crystallization attempts is often uncertain due to inherent features of the protein or to the crystallization process that are not fully under control of the experimentalist. The aim of this contribution is to propose user-friendly tools that can increase the success rate of a protein crytallization project. Different bioinformatic approaches to predict the crystallization feasibility (before any crystallization attempts are undertaken) are discussed and a novel approach to assess the nucleation process of a given protein is proposed. Practical examples illustrate these two points.

Cite this article as:

Sanchez-Puig Nuria, Sauter Claude, Lorber Bernard, Giege Richard and Moreno Abel, Predicting Protein Crystallizability and Nucleation, Protein & Peptide Letters 2012; 19 (7) . https://dx.doi.org/10.2174/092986612800793127

DOI https://dx.doi.org/10.2174/092986612800793127	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305