Abstract
This study investigates lysozyme immobilization on modified detonation nanodiamonds (MND) adapted for biomedical research purposes. Catalytic activity of lysozyme was evaluated after the enzyme was immobilized on MND through nonspecific adsorption or covalent binding. The enzyme adsorbed on MND particles or covalently conjugated to them exhibited catalytic activity and lysed Gram-positive and Gram-negative bacterial cells, but the activity of the immobilized enzyme was lower than the activity of the free lysozyme in both variants. The lysozyme covalently conjugated to MND was more resistant towards the proteolytic action of pepsin under acidic conditions than the adsorbed enzyme. The present study has only looked at a small proportion of the potential activity and applicability of associating lysozyme with MND. It therefore seems probable that further development will yield valuable insights.
Keywords: Bacterial cells, immobilization, lysozyme, nanodiamonds, MND, Lysozyme-MND system, Nonspecific Lysozyme Adsorption
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