Group A Streptococcal Vaccine Candidates based on the Conserved Conformational Epitope from M Protein

Title: Group A Streptococcal Vaccine Candidates based on the Conserved Conformational Epitope from M Protein

Volume: 1 Issue: 1

Author(s): Mariusz Skwarczynski, Abdullah A. H. Ahmad Fuaad, Lina Rustanti, Zyta M. Ziora, Mohammed Aqil, Michael R Batzloff, Michael F. Good and Istvan Toth

Affiliation:

Keywords: Epitope orientation, group A streptococcus, infectious disease, lipid core peptide, nanoparticles, self-adjuvanting, lipopeptide vaccine, N-TERMINUS J14 AZIDE, HUISGEN CYCLOADDITION, LCP CORE

Abstract: Copper catalysed azide alkyne Huisgen cycloaddition (click) reaction assisted conjugation of the multiple copy of conserve epitope (J14) derived from Group A Streptococcal M-protein to LCP core induced desired α-helical conformation of the epitope. The constructs were able to self assemble into large nanoparticles under aqueous conditions. Immunological assessment of these particles demonstrated their capacity to elicit high-titer systemic IgG antibodies against the epitope without help of adjuvant. The LCP systems incorporating the epitope via its N- or C-termini did not elicit significantly different immune responses.

Cite this article as:

Skwarczynski Mariusz, A. H. Ahmad Fuaad Abdullah, Rustanti Lina, M. Ziora Zyta, Aqil Mohammed, R Batzloff Michael, F. Good Michael and Toth Istvan, Group A Streptococcal Vaccine Candidates based on the Conserved Conformational Epitope from M Protein, Drug Delivery Letters 2011; 1 (1) . https://dx.doi.org/10.2174/2210304x11101010002