ESI-MS Studies of Silver Ion Competitive Interaction with Cysteine-Containing Peptides and Sulfur-Containing Amino Acids

Title: ESI-MS Studies of Silver Ion Competitive Interaction with Cysteine-Containing Peptides and Sulfur-Containing Amino Acids

Volume: 17 Issue: 11

Author(s): A. V. Novikov, R. A. Bublyaev, N. V. Krasnov, Yu. P. Kozmin and O. A. Mirgorodskaya

Affiliation:

Keywords: Sulfur-bearing amino acids, cysteine-bearing peptides, silver, affinity, ESI-O-TOF, amino, cysteine-bearing, argyria, bactericidal, (ESI-MS), isotopi, (Cys), (Hcy), (Met), Mass Spectrometry, AgNO3, TFA, D,L-homocysteine, HCy, P2, Ion Types, IAI RAS

Abstract: The paper deals with investigation of silver ion interaction with sulfur-bearing amino acids and cysteinebearing peptides using an electrospray ionisation orthogonal ion introduction time-of-flight (ESI-o-TOF) mass spectrometer. It has been shown that Cys and Hcy demonstrate the largest affinity for silver, both in relation to methionine and cysteine residues in peptides. The studies have for the first time revealed the effect of predominant forming of ions containing two silver atoms per a sulfhydryl group of cysteine-bearing peptides if the nearest microenvironment does not hinder this.

Cite this article as:

V. Novikov A., A. Bublyaev R., V. Krasnov N., P. Kozmin Yu. and A. Mirgorodskaya O., ESI-MS Studies of Silver Ion Competitive Interaction with Cysteine-Containing Peptides and Sulfur-Containing Amino Acids, Protein & Peptide Letters 2010; 17 (11) . https://dx.doi.org/10.2174/0929866511009011392

DOI https://dx.doi.org/10.2174/0929866511009011392	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305