Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution

Title: Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution

Volume: 16 Issue: 10

Author(s): U.T. Bornscheuer, D. Bottcher and M. Schmidt

Affiliation:

Abstract: In the past few years a considerable number of mutagenesis methods and high-throughput screening (HTS) systems have been developed and improved. In parallel, computer programs or software packages for molecular modeling have been further investigated. Thus, the number of examples for successful directed evolution and rational design is increasing constantly. In this review the essential mutagenesis methods and HTS systems, especially for esterases, are described and various examples for the application of these protein engineering tools are provided.

Cite this article as:

Bornscheuer U.T., Bottcher D. and Schmidt M., Protein Engineering of Carboxyl Esterases by Rational Design and Directed Evolution, Protein & Peptide Letters 2009; 16 (10) . https://dx.doi.org/10.2174/092986609789071216

DOI https://dx.doi.org/10.2174/092986609789071216	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305