Molecular Cloning of Grass Carp Growth Hormone Receptor and Its Functional Interaction with Silver Carp Growth Hormone

Title: Molecular Cloning of Grass Carp Growth Hormone Receptor and Its Functional Interaction with Silver Carp Growth Hormone

Volume: 16 Issue: 3

Author(s): Wei Hu, Xia Chen, Gusong Yu, Yan Chen, Yan Shen, Ying Zhang and Yi Gong

Affiliation:

Keywords: cell proliferation, CHO-K1 cell, grass carp, growth hormone level, growth hormone receptor

Abstract: A novel grass carp growth hormone receptor (gcGHR) cDNA was cloned by RT-PCR method. CHO-K1 cells were transfected with eukaryotic expression vector pcDNA3.0 containing gcGHR entire coding region, resulting in a stable CHO cell line expressing gcGHR (CHO-gcGHR). Bioactivity assay revealed that silver carp growth hormone (scGH) bound gcGHR with dissociation constant (Kd) value of 1.96 nM and stimulated CHO-gcGHR cells proliferation with the sensitivity of 20 ng/ml. The cells proliferation assay was also provided to measure fish growth hormone levels of pituitary and refolded protein.

Cite this article as:

Hu Wei, Chen Xia, Yu Gusong, Chen Yan, Shen Yan, Zhang Ying and Gong Yi, Molecular Cloning of Grass Carp Growth Hormone Receptor and Its Functional Interaction with Silver Carp Growth Hormone, Protein & Peptide Letters 2009; 16 (3) . https://dx.doi.org/10.2174/092986609787601679